XB-CLONE-4351933
Clone Name: IMAGE:7027209
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Gene:
ssr2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784576 [+]
taattaatcccccccccccccgcagttgagctgtggaagccggcaggatgaagtccatatttctggcgcttttgggcctgttggtggtggtgaggtgcga
ggaaggggcccggttgctggcgtccaagtctctgctgaacagatacgccgtggaagggaaagatctgactctgcagtacaacatctacaatgttggctcc
agtgctgcgctggaagtggagctgtccgacgactccttcccccctgaggattttgggattgtgtcgggaatgctgagcgtgaagtgggaccgaattgctc
ccgccagcaatgtctcgcacaccgtggttctcaggccgctgaaagctggatactttaacttcacctcagccacaatctcttacctggcccaggaaggcgg
tcaagtagtggtcggatacaccagcgcacctggacaaggagggatcctagcccagcgggaattcgacaggcgcttctccccacattttttggactgggca
gctttcggggtgatgactctcccatccatcggaatccccctcctcctgtggttctcgagcaagagaaagtatgatactcccaaacccaagaagaactaag
gcaaacccatcgaccggattggctccagccacatgtaaccccacccgactcgcagtcccagcggggatcgactcatccactgcgcatttgaacaaaatat
ccccccgttacgttgatctgggaccccccgggttatggccaaccggggtgttggcggcctcccgccttatgggaaactggaactttgtttccaactagaa
aaccgccccttggcccttttcccggtgcgccaaaaatacc
3' EST: CF784575 [+]tttttttttttttttattagaaaaccagtccctgataacacgcggctgtccaaatttgggaacatacaaaggcgacgtcctgtgtacattgaattccatg
tagtgagaggaatgctgcgggccgctagtgtacaacagaacgcagtctcggctctcacatgtaactgcatgtccaatagcaaaggggggtggaatatgaa
aatgataggaatttatttgtatttaaaaaatcctgtcctgccaatcacagcttgggatgtaccggctgcataattgcccaccaatcagatttaaaataat
atacatagaacccagaaaatagagatctataaggaaggatctggcagcgccccacccgaattgggcaacctgtatctactcccaatggcacatccagcac
ggagctcgggtaattggatcgtttaatcagaacatgatataaagggatatttgggacaggaagggcaaggcagttctagtagaacaaagtcagtaccata
agcagaggcaccacaccccgtttgccctacccggnggttccagatcacgtaacggcggatattttgtttcaatgcgcagtggatgagtcgatcccgctgt
gactgcgagtcggctgggctacatgtggcctgagccaatccggtcgatgggtttgcccttagtcttcttgggtttgggagtatcatactttctcttgctc
gagaacacaggagggagggggattccgatggatgggagagtcatcaccccgaaagctgcccagtccaaaaaatgtggggagaaacgccctgtcgaaattc
ccgctggggctagga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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