XB-CLONE-4351930
Clone Name: IMAGE:7027208
|
Gene:
bap1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784578 [+]
taattaatccccccccccgatagtctcggagacatggatatggcaacatgaataaaggctggctagagctggaaagcgatccaggactttttaccctgct
ggttgaagattttggtgtaaaaggggtgcaggttgaggagatctatgaccttcagagcaagtgcccagggccagtctatggctttatctttctattcaaa
tggatagaggagcgtcgatcgcgcaggaaagtctccactttgctggatgatacctccgttatggaagatgaagttgtcaacaacatgttctttgcccatc
agctgatccctaattcatgtgctacccacgcccttctgagtgtgttgctgaactgtagtggggttcacctggggcccacactgagccggatcaaggaatt
ctccaaaggattcagcccacagagcaaagggtatgcaattgggaatgctccggaactggcaaaagctcacaacagccatgccagaccagagccccgtcac
ctgcctgagaagcagaatgggatcagtgctgtccgcaccatggaagcttctcactttgtcagttatggttcctattaaaggacggccttttttgagcttg
atggcctttaaagtctaccccttttgaccatggccccctgggccgcaggatgaagaaattggacgaacaaaggcttcgcaacaattgatcaatcggagaa
ggattcgggactgggcctaacaccaaggggtaagcccgttatccatggaactatttaggtgttttaaacccttggattgggcccagttggcctgtgtcct
aatacatcccaaaagggcctttaaaaaaatatagtggaaagcgagccccagagccctttctcccaacaaattatttttttaaaaaaaaaaaaatggaaac
3' EST: CF784577 [+]tggtgtgcccggtggctctcctgggatactggagccttcagagcagcactctttgtttcttctgtgctctggccctccgtaggcttctgggcctgaatta
gttctggctgagttactcttatcaactgttgcaatgcttccagcacagtctgtctgttcatctttaaaatgtgaagcttgctctcatattttagccttcg
atctggcaccactgccatcaggttaaacctaatgtcatgatacggctcccctgctgtagccagtccgatcctctccatgatcactctgcgagccttgtct
gtccattcttcatcctctgcccaggggccatggtcaatagggtagactttaaggccatcaagctcaaaaagccgtcctttaataggaacataactgacaa
agtgaaaagcttccatggtgcggacagcactgatcccattctgcttctcaggcaggtgacggggctctggtctggcatggctgttgtgagcttttgccag
ttccggagcattcccaattgcataccctttgctctctgggctgaatcctttggtgaattccttgatccggctcagtgtgggccccangtgaaccccacta
cagttcagcaacacactcagaagggcgtgggtagcacatgaatttanggatcagctgatgggcaaagaacatgttgttgacaacttcatcttccataaac
gaagtatcatcccgccaagtggagactttttctgcgcgatcgacgctcctctatccatttgaatagaaagataaagccctagactggccctgggcacttg
ctctgaaagtcataaatc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: