XB-CLONE-4351924
Clone Name: IMAGE:7027206
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784582 [+]
taattaatccccccccccccaccccgccccagccctaggaagcttttggtgctgcttcttgagcctagcatctgctaacgtaacaccggcatcaccatga
ctgacgcagccatctcttttgccaaggacttcttggccggcggtgtggccgcagctatctccaagaccgctgtagcacctattgaaagagtcaagcttct
actgcaagtccaacatgcaagcaaacagatcaccgcagacaagcactacaagggcatcatggactgtgttgtcagaatccccaaagaacagggtttcatg
tctttttggcgtggtaaccttgccaacgtgatccgttatttcccaacccaggccctcaacttcgacttcaaagacaagtaccagaagatcttcctggata
atgcacctcagaataccccgccctggcgttacttttgctggaaccttgcttctggcggtgctgccgggtgaaactccctctggctttgtccaccccccag
actcttcccgtaacacttctaccccctgaagttgggtaaaaggcaaggttttaaaaaacaattctttgccccaacagcgaattggattaagttactaatt
ccccagaatccataattggccattaaaaaggaccttatattttatagccaatcccaactacaaataacgctctcttggtgtccccaatccgacccattat
ccaaacataggccataaatattttcgcggattctctttggaaaatcaagatttacaacaggtnaaggccttttttatggaatcaaccacaaaaaaacacg
cgcgcccttatttctcctaatcttccccactttgatttaatctgtatttcaccacatattattatacccacgccgcctcccatatttgtgatccaccccc
cccatttcataatatcccaccattttgccccccccacaacaatataaataatattgtccccagcaattgaccatcataattan
3' EST: CF784581 [+]tattttttatttcctcaaacctaagttacttaaaaacatgaatgactgaaataaaaaataaaggagcgttgagtcccactgaatcagcctgtcactgcca
aagggccaaaaactgccaaggaggcacagttgcaggcactaaaagcagtaagagacttaaagggaatgatttattaatgtggaaagctggtgagatgtaa
tagaactgatcccaaaataccagccagtaaagattaccagctatattttataaataagggtgtgtttgacaaaaaaaaataaagaaggtccatgaatgtt
caaggtatgttacataatacagcatgccaggtcacagacatctcaacgaggacaagtttagatgtatttcttcagctcatcatacaacaccagcacaaaa
gcgccacccattcctctgagaacattggaccaggcacccttgaagaaagccctgctgccctcatcccgtgcaatcttcctccagcagtcaattgttccac
tgtacatgatgtcagctcctttccttccagactgcatcatcatacgacgacgtactgtgtcaaatggataggagacaaatccagctaccgctgttacagt
ctgagcaatcatccagctgatgataatgtgtgtgttcttgggatctggaagcatacctttagctgtatcatagatgccaaaataagcagctctgtagatg
atgatgccctgaacagatacgttgaaatccctggtacaagccccttgatcccctcagatctgggagatcttaactaagcaatccccca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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