XB-CLONE-4351919
Clone Name: IMAGE:7027204
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Gene:
gcn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784585 [+]
taattaatcttctccctcccctggagtgttaaagtggctctgcttgaaaccctgagccttcttctggccaaggttggcattgctttaaagccttttcttc
cacaactgcaaaccacatttaccaaagctctgcaagattccaaccgtgctgtaaggctcaaggcagctgatgctttgggaaagctcatttgcattcacac
taaagtagatccacttttcacagagctgcaaaacactatccgtgtgtgtgaggactctggagtcagggaaaccatgctgcaggctgtccgattcattatt
cagggtggtggaggaaagatagatggagcaatcaggaaaagctatgttaccttgttgcttgggatgcttggacatgatgaggatgccacccgcatggctt
ctgctggctgtcttggtgagctgtgtgcttacctacctgatgaagaactttctgtactactacagcancatctcttagccgattttactggaattgattg
gatggtaaggcgtggaaaaaacatggcatttgctgttgctaataatgttgcatccagaaaggtttgcaccccacagtttaaatataaatattgaaacccg
tgggcttcaactaaggccaacagcaaactgggattttcattaacccactgaagtgaaaacccaaggggatgggaatttcctttatgtaaaaatttaaatt
tgaagggcgaaaaaatggaaaacctttgcccccccaaaaaccttttaaccccttttctcctgaaatacggcctttctaaaaccaccccggcatttgtaac
cctacaaccctttgggggggcgctaacaccccatgttaattcttggttgggggcctaaaacatcaaaata
3' EST: CF784584 [+]tttttttttttttttcgctattttaaaggtgtgtatttttttacaacaaaccaatgttgaaaatgattccaaaattttctgtccgaggccttagatctac
ccatggggcaataaaaatatctgtctccttcaggtttcacagtgaatgtcatgtaataaaacaaacgcaaacaatctacagtccctgtaatcttttttta
cagttgggtttaattttgcatttgtgcatttactctttattgggcaaatagcattcatttttttccattatacaaattttcaacactaatcttccaggtg
tttaaccattttttcacaagcatcacttttatctatgcagtaactaaattactggttggagatgtggtatagaaactgatttttaattattcttttaatt
aaaaagaaacaatggtaagccaatattcctgcactacccaacgagtgcattttcaaaaacgaaaccattaaatgaatgttcagacatgtaacaatcatac
aagcacatggtctccatcacgttagaatagtttcatcaatctgttcagtagagtcagcctgagctgcaatcttcttgagagatctgcgggagctttcatt
caacatctctaaactagcagtatccagtatctttgtaatattctggaataggtcattgcctgccctcattttcagcaggttgactatggcctggtcgctg
taggcccgcacactggnatttttgtcttttggattggccaaaaaacacttttgaaaattggtttgataagcctgggaggtctaaaagg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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