XB-CLONE-4351913
Clone Name: IMAGE:7027200
|
Gene:
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784588 [+]
taattaatcccccccccccccgcatctttgcactctgctttgtcctagctgacttgtcggagaatatctaccaataaacagccatcatgacttcctatag
gtccagctctgcttcatactattcaagctcatccggcaaaggtggctttggaggcagcagctatggaggcagcagctttggaggaggctttggcagcggt
gcaggctccggcttctcatcctctgggggtaactttgccatggcggcggcagacagttccaacttaagcaacttcggtggaaatgaaaagcacgccatgc
agaacctcaacgaccgtcttgcttcctacctggacaaagtaaaagcactggaagcctccaatgtcaccctggaagggaagatccatgactggtatgatag
caaagtggatgccggcattggagctgggtctaaagattacagcaaatattttgagatcatcaatgagctgaggagcaagattggtgccgcaactgttggt
aacgctacagtgaccctgcaaattgacaatgccaaactggcggctgacgacttcagaattaagtttgagaatgagctggctcttccccaaagcgtggaag
ctgatatttggtggcctgcgataaattcctggaatgaactgaaccttggctaaagggggacctggaaaatccacaacgaaaaccttggaatgaaagacct
gggccctacctgaaaaaaaaaaaccttggggatggaaaatgatttcctgtcaaaaaagcccagaaccggccggggaaattggtagatgtcgaa
3' EST: CF784589 [+]tttttttttttttttagaatctaagggaacattcctgttcatttgtgcctttatctttcattgccttggttttattcaattctggatgaaacaaccttcc
catctaccacctcctcgacaatggttttcaccattgtggttctagtggttgatgattctgttttggactccacggaacttgccttggccacggtggtcat
ctgtcccagttccccttccagcaggcgcctgtatgtttcaatctccatctccagcctggtcttaatgtcaaggagctgcttgtactccatgttctgatgt
tccatgtcggacctgatctgcagaagctgctcctctaggctgctgatagtgttctggatctgtagaagttgcgctgaatagaagccttgtacttcactga
ggttgccttcaactgattgtttcattgccagctgggattgcagctcaatttccaagctctgaagtgaccgtttcagctcggtgatttcgctcttgctggc
ctgcacttgttcaaccccaactgaaatctctttcttcagctcaccgctcttctggttgaaaccaaactctgcatccctacggttcttctcagccagtatt
tcatagtcacctctcatgttattcagaaagttggtcaaatccccccccgggagccccgtccatcttcaaacttcccttttcccggcgcctctggcttctt
ttgcatggactcaatctccctccccaatggggtccttctttaag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: