XB-CLONE-4351910
Clone Name: IMAGE:7027199
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Gene:
gnb1l
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784591 [+]
taattaatccccccccccccccgacttgtggcgccgggattagctgtcagactgactggcgtttagtttaatacatgtgacctgggtggaaaaaacagca
cagattactactgtggaacaattgtactcaaaatggctcttccatctcctgatccaaagtttgaccttcgggggatcgatgcagaggtgaactctttgca
ttttagttgtatggttcatagaccaagtcaaccacttctcttctcagggtcctcaaatggtcttgtccatgtgtggaatttaaatactcggagatctgag
acagtgctcagtggtcatcaggggaaatccatctactggctccatacactgtttcataacagggaattgttgttaagccagggcagagaccttcaaatcc
gtacatggaatttagcagaaggcagaagggaagaaattgactctgttcctgtggagagtgttggattctgtaaatgttctttactgaagactgaaagttg
ccttttagctatgccaggaaaggacagttcacaggtccagggttttggatcttaattccaaaaagatagtctcctcaatgaagctcagcacagataacca
atggggtatggctatgtgcatgaagttatggcagcccatctctggtccaagccccctttcttctttgttgctatgaggatgggctcaatggctcctgtgg
gaatggttttggaaacacagaattgaatgaagccgaactccctttgcccacaatggaagccctgntgaagaagccctttgacttttgaactgccctt
3' EST: CF784590 [+]tttttttttttttttattagacttatgattttgaagtcaatattacatgagaacaaatcgacataatagatcccatacctgtatactttatgtaaaataa
aaacagaattggtacaggtatgaaatcaatttactgtaaacagcaaaaataggacatgtcctctccctaaaactggaaacccacaatcagaacacaaaca
attcttcatttggatgatttgctttctgtatggaagacagtgccttgtacttgatatatactaaggtagaacaaatcttaacagccttagcgtatagtgg
tccatatccaaacagagtcagcgtaatagattgtaaggtacccaaccctcattttaacaccagtcctttatgagagtccatttccatcctttcatggtct
caaaacttggttatcgatacacaacttctttgtcatctgcttcaaccagctggtataattccaagaaacaaaagtattgcataccatgactggtttttgt
ttcctcagtcgatgaccattttcttttaaaatacagtcttatccaaaataggactggatttaagtaaaagtatactgaaatgttgtgtatacacagggta
tgtttgagaatataaagtatcttaggtgttctgatatactgaccataaacttataagatggtcttttgagccagctgcaagcaatcgatcttcaggggtt
aaatggtctgaaaaagacacacagtgcccaataaccgaagtgaaactgtaacacaacctaaaaggtttcttcccgcttcccccccgaaatactccaaatt
ccaatggggccccaacccttggcagtaacctaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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