XB-CLONE-4351905
Clone Name: IMAGE:7027196
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Gene:
timm21
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784593 [+]
taattaatccccccccccccccactttagtcagtttacacaaagcactgataatgcacggactgtgatgtatgtgttggcaagaggggtacaatgatgtt
gcctcggtgtttgcggagagcggtcctgtgtagttctagggcactggggtcacctcgatccctgactgtaccctgttacagaaacctctcatgtctgcta
ctttcccagcagcgggcccttgctgtgccaacaagccggttctgtgctgagcggcccataagcacgggcagtgctttgctacaagtgaaggacaagcgag
tgtctgtgcaaagcagaggtgacggggcgtctccacaaactgcttctcataaagtaaaagaagctggcagagacttcacatattttattgtggtactgat
tggaatcggcgttacaggaggacttttctatgttgtctttgaagaacttttttcttcttcaagtccaagtaaaatatatggagaggctcttgaaaagtgc
agatctcatccagaggttattggtgcttttggtgagcccatcaaaggttacggagagactacaagacgtggtagaagacagcatgtcagtcacatggaag
tatgtgaaaaatggcataaagtgcatgaggctaaagttctacatagaaggcttggaaaccagaaaaccagggaacagtgcacacagaaggtggaaagaga
acccaaaaagtgggaaaataatgaattttcagttacttttttgtttgaaatttgatactttaccccacggcggaaaaattccttcataag
3' EST: CF784594 [+]tttttttttttttttgttttttgttttatttttaaattacatgattcatttattttcagaataaaaagaagccatgattcagtttctcacagacatttga
actgaaggccctgcctacagcaaaaccttctaacattacaggaaaccacacagttgtaaagcaccttttatacagatatagcagcggcaaacaatgattt
tcttcttaactttggcgtctgttgtcttctatgacgattgttcgccgtgggtaagtatcaatttcaacaaatatgtactgaaattcatattttccacttt
ctgggttctctttcacctctgtgtgcactgttccttgctttcttggttccaagccctctatgtagaactttagcctcatgcactttatgccatctttcac
atactccatgtgactgacatgctgtcttctaccacgtcttgtagtctctccgtaacctttgatgggctcaccaaaagcaccaataacctctggatgagat
ctgcacttttcaagagcctctccatatattttacttggacttgaagaagaaaaaagttcttctaagacaacatagaaaagtcctccctgtaacgcccgat
tccaatcagttaccaccaataaaattatgtgaaggtctctggccgccttcttttacttttaagaaaaaccgttttgtggaaaacgccccgttcaccttcg
ggctttgcacaaaaaacctccccttggtccctcccctttgtgaacaaaagaactggccccggggctttatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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