XB-CLONE-4351902
Clone Name: IMAGE:7027194
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Gene:
mrps2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784595 [+]
taattaatcccccccccccccgcagtgaccttccccttatgggactcagcatggcggcgtctggcctcaccgggctgataaagagagtattccccaggct
cggtgctgccagtctccagtcgccagtcggtatcgggccggccccttacccagaagcacttgggagatgttacggcgcagcccccgcggctcagcagcac
ccagagcccagccccgcggacttcacccagaagcttttagtggaacctttaaagcacccggacttcttcaatgtgaaagaactgttctccctgagggaat
tgtatgattccagagtccatctggggcacaagaagggatgtcgccatcggctgatggaaccctacctgtttgggtgccgcttggaacaggatataataga
cctggatcagacaatgaggcacctacagttggcgctgaacgtcaccgcccacatcgcttaccggaaaggcgtgatcttgttcgtgagccgtaaccggcag
ttcagccatctgatcgagagcaccgccagggactgtggggaatacgcacacacacgctactggcagggggggctgctcactaacgcccccgtccaatacg
gcgccggcgtccgactcccggacctgatcgtcttcctcagcaccctgaacaccgtcttcgagcaacacgtgggcgatccgcgacgcggccaaaatgaaaa
tccccacggtggggggtagtcgacaccaactgcaaccccggcccttcatcacttaccccaatcccgggcaaa
3' EST: CF784596 [+]tttttttttttttttgaatatatacatttattaggcaattacacgaaaccaaaaaaaagtcctttttacagtgaatccccagggggaggggcttctgcag
gggggaggggctacgcctcggagattttgctctgcagcccctggagcagctccatgtgtttgcgcttctctttggcccggttaatggtcattttaaagag
gcggcagtagagttccatggcgggggcggagtcgtcgttgccggggatggggtaagtgatgaggccggggttgcagttggtgtcgactacccccaccgtg
gggatgttcatcttggccgcgtcgcggatcgccacgtgttgctcgaagacggtgttcagggtgctgaggaagacgatcaggtccgggagtcggacgccgg
cgccgtattggacgggggcgttagtgagcagccccccctgccagtagcgtgtgtgtgcgtattccccacagtccctggcggtgctctcgatcagatggct
gaactgccggttacggctcacgaacaagatcacgcctttccggtaagcgatgtgggcggtgacgttcagcgccaactgtaggtgcctcattgtctgatcc
cggtctattatatcctgttcccagcggcaccccaaacggtagggttccatccgccgagggcgaaatcccttcttggggccccgatgggactctggaatcc
ataaaatttccctcaggggaaaacaggttcttttcacatttgaaaaaaaatcccgggttgctc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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