XB-CLONE-4351893
Clone Name: IMAGE:7027190
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Gene:
slc1a3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784601 [+]
taattaatcccccccttcatacccccccgatgtatgagtgctccctctcctgtgggtgcttctgctgcctaacgctccacatcccatcccctgtctccct
gtccacacaaagttcttgctactccgcctactccctttcattatccactggaagttttagcagctatttaatcccaacaagaggcatttgaaggaaaaca
agaagatgactaaaagcaacggggaagatccacggtcagggaacaggatggagcggtttcaggaaggggttcgccagcgttcactgaaaggccaaaaaaa
aaatgcggaacataacaaaagacgatgtgaagggtttcctccagcggaaagggttttctccttttttaaagcctttggctggccattaattgggtattaa
cccgggggaatttttttgttcccaaaatttttccctttggacactttgggggaaatttttaaaggtatttttttccccgtttcccaagggggggaaggtt
ttctaccctagtaaaaaaaagggcgccaaaaaaaaactcggggccccctcccccccccctaatatggggttaacccccccacctttattatattggacat
cgggaataatggggggattgccacttcctggggggccaacgtatgaaggggggtggtatattcatgggggcgaaagaaattgagggggcgtttatatata
aaacaaaactctgtcgtgggggattaaa
3' EST: CF784602 [+]tttttttttttttttggggacattcagatacacactaaagtcactggtgacacaaagcaatggtttctatggaacataatgatgaactgcaagctgcaat
aataacaagtgtcacattcagtgtttgcattacaatgctttgtgagttctgaagcatcgggcttcagaaatttctgtccagtgtcaaatccaacgctaga
gtatgtaacatggaccaactgatccttatcatattccctagaaagtggttacttagcagcctttatggacccctgtgtaaatacatgttccttgtctgtc
ttaaagaggaaatatttttgtttatttgatattttaggtaaaagccaaggagctgtggagcaaatgcctgaaaacaatggaagtaacactttaatacttt
agtaaatggggagagtaaagtttacaagattgtttgagtgaaattgttatgcagcagtgttgatctagcaacgtgataccgaatgtggaaacccccatct
acattccctacatttaacacctttttgtgcaaggaaaggctgaatgcagttatttatctgtttccaataaaacagaatgaagatatatattgtattgatt
ggggattttagcagctgctaattgtcattgtgttggggtgggtgttcctgcaatcacacctggagtgcaanaagggaagtggccatgacttgggttttct
ttttagacaaagaaaaaaaaagtggggatggtgaatgggattctttttacattttttggtttcactggcccaaaagggcttctccgggctttcttttttc
cttggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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