XB-CLONE-4351890
Clone Name: IMAGE:7027189
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784604 [+]
taattaatcccccccccccccaggagcatttggagctttgtgtggcagatatcccagagttgttatctgctaatcaggtgaagttaaatcagtaaagcaa
gtaaatcaggacctacttttgggcggaacatcttaatacacccagatgctacacccaagagaaaggtctctagagggttactatactgataaatatttta
ctgtaaaaaataacttttgcaaaaagtgaaaatatgacttttccatatccctatacatagtatatacactactgctatacactatacagaaaaaaacaat
aatttgcccacactccatggggaaaattaacactagaaccctgtcaagctttatatagacttcctgtattagtgtgaatgacaagggattaagttgttaa
tgtggcttgttaggcaagtttattttaactaccatactggcaaatgccaatgcaaattattaggtgggactgaaagtaagcatctagatgaaatatgacc
ctatggattagatgggccaggttttcacatgttgaggaatttttcatgataattatcacaagtattatttgctttaaataattctgttcagcattaatgc
ctattgtagcctgtttttaatttttaaactctgtatggtttgacaagtgtgtattacaatattaaacgggtctttgcttttgatttttggttccccatat
agatctctaataagtggaaagtatccttaaatagtgtttgttacctttcaaaccagcatctgttgagggtaattactggtccaaaataaaaactggtagt
tgtaagtcaa
3' EST: CF784603 [+]tttttttttttttttgataatttggcagaaagaaagatgaaatcaaattgatttcaaatctttgtaaaatatattatacataaaaatggctatagtaaaa
taaaggcagccaacatagttggttacagttgcaaaaaggcaaataaatggtagaatgattggtttataagtcaatgatcagtaaagtaaatttgggagcc
catcccccccatcaaaattttattttcagccccccactacttatcacttaaaatcacgggtttcccatggtcccacaaaacccatgggttagctcatgag
ctcccctgcagatcagggtctgacccctctaaagataagccactgactactatggaacacaaaaaaggaacaaactagatttctgcatgcctggctaagt
tatttccctctatggtctcttactattttccccaaagaattcatgtacatcgcttgcaaatgcaagctgaaacatttatacagtagcagagcagaggaac
agacaatggtgacacttattcatctctgtggagttgcaaagtaatcatttgtacatggaaatgtcctgcaatacacaggatgctactttacttttaaaaa
aaaagtttgctttggtgttttactataaaaatctgattgtattaaatcgaatcagtgtcccagaggaaaacacagcctgactgtactttgcagtgaaaat
tacaacttgcttgaaatacatatttatccagcccactgtagtttgtgcacagcacaatgtgctttgcagacaacacaatcctttttctatcctactaagg
caagcaacagggggcatcat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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