XB-CLONE-4351887
Clone Name: IMAGE:7027187
|
Gene:
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784606 [+]
taattaatcccccccccccccgatcccagggaccctgagaagaacaagatgaaagccattatcctgctcgctctgctagccgccgtcctgattcaggata
taagtgcagctccggtgcagcctgagggaacagagcctaagagtcctcttcaccgtagaggttgcgttatctggtcctgctttggaaaaagagatacaga
caaacctgaggcaaatggggaaggtgaagctctggtgaaacctgaggaaactgatataagtgcagctgcggtgcagcctgagggaacagatataagtgaa
gctctggagcagcctgaggaaactggggaaggtgaagctctggtgaaacctgaggaaactgatataagtgcagctgcggtgcagcctgagggaacagata
taagtgaagctctggagcagcctgaggaaactgagcgtaagagtcgtcttcaccgtagaggttgcgttctctggttctgctttggaaaaagagatacaga
caaacctgaggcaaatgtggaaggtgaagctctggtgaaacctgatgaaactgatataagtgcagctccggtgcagcctgagggaacagatataattgca
gctccggtgcagcctgagggaacagatataagtgaagctctggtgaaacctgaagggaattggtcgtagatccctccacgtcgaaagtatgtataggttc
ctatgcccaatgggggattaagaatctttcaacaaaatgttgatgtga
3' EST: CF784605 [+]tttttttttttttttggttgaggacatttaatggaaaataaacatttaaagtccatggctcaggcttttccttagcttcacatctgaaatcacatcaaca
tttgttgaagattctaatccccatttggcataggaacctatacatactttcgacgttgagggatctacgaccaattccctcaggcttcaccagagcttca
cttatatctgttccctcaggctgcaccggagctgcacttatatctgttccctcaggctgcaccggagctgcacttatatcagtttcatcaggtttcacca
gagcttcaccttccacatttgcctcaggtttgtctgtatctctttttccaaagcagaaccagagaacgcaacctctacggtgaagacgactcttacgctc
agtttcctcaggctgctccagagcttcacttatatctgttccctcaggctgcaccgcagctgcacttatatcagtttcctcaggtttcaccagagcttca
ccttccccagtttcctcaggctgctccagagcttcacttatatctgttccctcaggctgcaccgcagctgcacttatatcaatttcctcaggtttcacca
gagcttcaccttccccatttgcctcaggtttgtctgtatctctttttccaagcaggaccagataacgcaacctctacggtgaagaagactcttangctct
gttcccctcaggctgcacggagctgcacttatatcctgaatcaggacggcggctagcagagcgagcaggataatggctttcatcttgttcttctcagggt
ccctgggatcgggggggggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: