XB-CLONE-4351840
Clone Name: IMAGE:7027170
|
Gene:
eef1a1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784636 [+]
taattaatccccccccccccccgctcttcctcaccgggtctgcggcgagttttaagtgtccaccgccaaacatctaacaatccacaatgggaaaggaaaa
gactcacatcaacatcgtcgtcattggacacgtagattctggaaagtccacaacaaccggacatcttatctacaaatgtggtggtatcgacaagagaacc
atcgaaaagtttgagaaggaagctgctgagatgggcaagggctccttcaagtacgcctgggtcttggacaaactgaaggccgagcgtgaacgtggtatca
ccattgacatctccctgtggaaatttgagaccagcaaatattatgttactatcattgatgctccaggacacagagacttcatcaagaacatgatcactgg
tacctctcaggctgactgtgctgtcctgattgttgctgctggtgttggtgaatttgaagctggtatctcaaagaacggacaaactcgtgagcatgccctc
cttgcctacactctgggagtaaagcaactgatcgttggtgttaacaaaatggattcaactgaacccccatacagccagaaaagatatgaggaaatcgtta
aggaagtcagcacatacatcaagaagattggttacaaccctgacactgttgcctttgtacctatttctggatggaacggtgacaacatgcttgagcccag
cgccaatatgcctttggtttaaagggtgggaaaatctcancgtaaagaggggatctggcaaccggaactaccctgctggaag
3' EST: CF784635 [+]tttttttttttttttcttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttaccctt
aaacttttttttaaggggccccatttggggaaaaaatttgggcaaattccccccctttaaaaaattcgggggttaaaaaccaaaaaaattaaaaaccccc
ccaaaaaaagggggccccaaaaatttttttccccccaaaacttttgggaaggctttaaaaacaaaacccccctttgaaaaaaaaaccccgggggcccaat
taaaaaaaaaattttaaaaacccccccccccccccggtttaaaacggggggaaaaaaccgggaaaaaattttttgccctttgggggtttcaaaaaaaaat
tttggaattttcccccccccccccccctttttaaaacccccttgaaaaaccccaaaaaaaaaattccccccttggtcgggaaaaaaaaaaaaaaaaaagg
ggggggaaaaaaaaaacccccccccccaaaagggtttccgggggatttggccaaaagggggcccccccccaaactcggaaaaggggggggggcccccctt
tttcaaaaaaaaaggggaaaaatttttttccccccccccaaaaaatttaaaaaaagggggggggggggggaaacccccaaggggggaaatccccccccca
tttggggggggggggtaaaaaaaaaaccccggggcgggaaaagagcacccccccccaggggggggggttttttggggggccccacaaaaaatttttcccc
aaaaaaggaatcctctcaaaaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: