XB-CLONE-4351828
Clone Name: IMAGE:7027166
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Gene:
ocm4.3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784644 [+]
taattaatcccccccccccccgacacatctgtcacaggaagactgcttgtatctcttcactccagctacagaggacttcggactgaccatggcattcggt
ggtatcctgagtgatgctgacatctctgctgctctgaagaactgccaagctcctgactccttcaacttcaaaactttctttacccagtctggcctgagca
gcaagtccgacaatgatgtgaggaacgtctttgccatcattgaccaggacaagagcggcttcattgaggaagatgaactgaaactgttcctccagaactt
cagcgcaggtgccagggctttaaccgatgctgagaccaaggccttcctgaatgctggtgactctgatggtgatggcaaaattggagttgatgaattccag
tctctggtgaaaaactaaactttttgttaaaatctgaatctgtgaaacatcacaaaagatgaatgcgggttttctgcgttaatctgatccaagtgaaacc
tggtttggcggaacacagacgtctcatcaatatttctccccctcagaaaagtggtgtcatcatctggtggcgggattcatttatttattcacaaaaaagc
agttgtttttcttcaacattttgtgaacccagaaatggnttacccttgcctacagtttatcaaacagccccttaatgaaaccaacatatttattctcatt
tttatactaataacattgtccattgtttacactgttgagacttttgcaaatccataaacgtgcaatttcttgctacagttttccctatgggactgagcag
gcataaaaaaattgttt
3' EST: CF784643 [+]tttttttttttttttgattccaaaattgatttattaagaacaatttttttatgcctgcctcagtccataggaaactgtagcaagaattgcacgtttatgg
atttgcaaaagtctcaacagtgtaaacaatggacaatgttattagtataaaaatgagaataaatatgttggtttcattaaggggctgtttgataaactgt
aggcaagggtaaccatttctgggttcacaaaatgttgaagaaaaacaactgcttttttgtgaataaataaatgaatcccgccaccagatgatgacaccac
ttttctgagggggagaaatattgatgagacgtctgtgttccgccaaaccaggtttcacttggatcagattaacgcagaaaacccgcattcatcttttgtg
atgtttcacagattcagattttaacaaaaagtttagtttttcaccagagactggaattcatcaactccaattttgccatcaccatcagagtcaccagcat
tcaggaaggccttggtctcagcatcggttaaagccctggcacctgcgctgaagttctggaggaacagtttcagttcatcttcctcaatgaagccgctctt
gtcctggtcaatgatggcaaagacgttcctcacatcattgtcggacttgctgctcangccagactgggtaaagaaagttttgaagttgaaggagtcagga
gcttggcagttcttcagagcagcagagatgtcagcatcactcaggataccaccgaatgccatggtcagtccgaagtctctgtagctggagtgaagagata
cagcagtcttctgtgacgagtgcngg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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