XB-CLONE-4351825
Clone Name: IMAGE:7027165
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Gene:
h3-3b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784646
[+]
taattaatcccccccccccccgacgttagtttgtctcctcgtttgactgtttgggttacgctcccgggagtgatcgaataacaagaagcaatggctcgta
caaagcaaactgcccgtaaatccactggtggaaaggctccaaggaagcagctggctacaaaggctgccaggaaaagtgctccttccacaggtggtgtgaa
gaagccacacagatacaggcctggcactgttgccttgagagaaatcagaagatatcagaagtctactgagctgttgatccgaaaactaccattccagcga
cttgtaagggagattgcccaagacttcaagactgatttgaggttccagagtgcagctattggtgctcttcaggaaacaagtgaggcttacctggttggcc
tctttgaagataccaacctgtgtgccattcatgccaagagagtaacaattatgcccaaggacatccagttagcaagaagaatccgtgggcgagcgtgctt
taaacattttgctaggaaactttttttttaacttagtttttttttttttacaattgttactacaagggggggttgcccttttattttataaaaggggttt
gtgttacctggtgaattaactttaaccgtaaagatgggtaataaaaacatttttaaaaggaaatttccccttaaatccccccaggttttttttaaaaaaa
ttttggtattttcgcaagccataagtctttttttgtgaacccccctctcttttttaaaacacctaattggccaggcgccaaaaaactgcaaatcttttgt
ataccataaattttaaaatattttcttttccggggggaaaatttccctcttgtggggaaataaaacct
3' EST: CF784645
[+]tttttttttttttttctgaggtagtggaaagtttatttacaatggagttacacagaaagatactaaattatgtaaagaatgaagtatgggcatgcactat
gttttaaaggagagggtcacaaaagactagctgcagatacaaatttctgaaaaacctgaggattaagggaatgtcatataaaatgtttactaccatctta
ctgtcaactactctacagttaacaaaccctttataaaataaaagacaacacacttgtatgtacaattgtaaaaaaaaaaactaagttcaaaaaaaagttc
ctagcaaaatgtttaagcacgctcgccacggattcttcttgctaactggatgtccttgggcataattgttactctcttggcatgaatggcacacaggttg
gtatcttcaaagaggccaaccaggtaagcctcacttgcttcctgaagagcaccaatagctgcactctggaacctcaaatcagtcttgaagtcttgggcaa
tctcccttacaagtcgctggaatggtagttttcggatcaacagctcagtagacttctgatatcttctgatttctctcaaggcaacagtgccaggcctgta
tctgtgtggcttcttcacaccacctgtggaaagagcacttttcctggcagcctttgtagccagctgcttccttggagcctttccaccagtggatttacgg
gcagtttgctttgtacgagccattgcttcttgttattc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: