XB-CLONE-4351822
Clone Name: IMAGE:7027163
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Gene:
znf217
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784648 [+]
taattaatccccccccccgatttactatcagatcatctacaggcactaactgcctgctcactgctgggactccttctgctgcccctgatccggttcgagc
agatcactttatgaagatgtttggatattgatattaacgatagctgctttgagtagtacatgaaccaatgccatgcttgtgagctacagccatgcttctg
catgcattgtgatttctaaggtttcctaaaggggagtcttaaaccattttgttttccacaaatttttcaacgtccagattaaaaatgcccgtccagtcat
tttccgatcttgattgcccacatggggtgggcagtgccattggttctcagatggacagtcctgattcttccctaacccccaaacggcactacctccactt
ctacacaataacctgcccgacaccttttctcatgcctgtcttcatccacacattgttaatttctacccgcacgctttttttttacctttcccctattcct
accttccttgaacggatcctattgccccgcctatagctcctctgggcccccagtgcttcatcgcccttatatcttttcctttatgaatcccacccaccgt
tacccctatgtttgttttcgcatttgcacccaaatttttccttcatcttactccccccacgccttttaacgatttgtctcccatatcacatgttttctcc
ccgcctccgcctttcttcgcccagaattattcaacgtctgccccttcattcgcccatccaatgcggtacgggcgtcctttgcaccttggttgccccggcc
acctcttatgatcgcagcaacccctcttgtggatcatatttactggggattcacttttgtgcggagcctccgtaacctttgcaattataaaggaaatcca
cactagttgaccctcctcacccattctgcttatgtcttcccgccaccaccctctttttttgcgcgcctgtgagtacaaaattcgcacatcttcgcttcac
gaatatcctttccacagtgaagacaatatctaattctgtccccctatatcgcggtatttctaccgcctcccgcgcaataacgcgct
3' EST: CF784647 [+]tttttttttttttttgttttttttgacaacactatattttttcaacagaaatgaatgttggaagaatatgggaaattgccacgcatacagtttacagata
catatcttctctccccctcccttggaaaacggcagggatatgaaacagaaataataataatacagtgtctcccctttaatttggtctcttgggaaaaaca
acattccaaaaatagatatatttatatataaatatacacaaaacattctataacttttataatacagtatatctgcaacagaaacaaaattcaaagggaa
aagccaaaataacttgtgttctagtgttctttgtagaaacaatccactttgttttcagtagatttcagaaaggaattccatagaaagtccccagaaactg
gatcattattaaactggaaaaatctaggctttgtcatttggtccaaacccccgctttgatacatgtcggtttataggtgcttttcttccttctgttacgg
aggaaggctcctgactgaaggaaagatctagtactctgtaagttggtcctgtggttggaggaacacgaatgattttgtctccaacgtgattccacagcat
gtggactactcacacccatgaggccacaaaggggcttccaaaatttttaacaggattttttcctcggaaaaaggaagcttagcaagtttttgagtatggg
agggttttgggcacccacattggttcatttgttgggggaaccgggtaaaaatcccccaataagggtggattttgggctctaagcccttctttgaataagg
gcctcttcgcacttttctaaaggggggaatggtttaaggaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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