XB-CLONE-4351819
Clone Name: IMAGE:7027162
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Gene:
upp1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084518 [+]
ATTCTAGTGGCAGCCGACCGTCTGGCCCTGTACTGGTGTGTTGGGCAGTGAGAGCCTGGAACGTAACTGGGAACACAAACAGGACAGAAGAGCCAGCAAG
GAGCAAGTGGGAGGGAAGTCGGACAGGTAACAAGGGTGAGCTGCTCTGGAAGAAGGAGATCCAAAATGGCACCTGTGCATACTGCTAAGAAAACAGAAAA
TGATCAAGCCACATCAAATCTTGTCCACGTTGATAATCCACACCTAGAGAAAATGAAAGAAGACATTTTGTACCATTTTGACATTGGAACTGGGACTCAT
GATTTTCCTGCTATGTTTGGCGATGTGAAGTTTGTCTGTGTTGGAGGAAGCCCATCCAGGATGAAAGCATTTGCACAGTACATAAATGGAGAGTTGGCAT
TGGGTGACCCCAACTCTGATGTTGCCAATATCTGTGCAGGAACTGATCGCTATGCAATGTATAAAGTTGGACCTGTCTTATCTATCAGTCATGGCATGGG
AATTCCATCAATATCTATTATGCTCCATGAATTGATAAAGCTGCTGTATCATTCCAAATGCACCAACGTCACGATTATTCGCATTGGCACTTGTGGTGGT
ATAGGTTTGGAACCTGGTTCTGTGGTAATAACAAGTCAGTCTGTGGATGCTTCATTTAGACCTCAGTTTGAACAGATTATCCTTGGAAAAACTGTAGTTC
GGAACACAGAGTTGGATTCTGAGCTTGCCCGAGAATTCTTTCAGTGTAGCAAAGAGATCAACGAGTTCAACACAGTCATTGGAAATACAATGTGCACCTT
GGATTTTTATGAAGGTCAAGCTCGCCTTGATGGAGCATTTTGTTCATATACCGAGGAAGAAAAAATGCAGTACTTGAAAGCAGCATATGATGCAGGAGTA
AGAAATATTGAAATGGAGTCTTCTGTGTTTGCAGCGATGTGCAATATAAGTGGACTGAAAGCTGCCGTAGTATGTGTTACACTTTTGAACCGTCTGGAAG
GTGACCAGATCAGTAGCTCACATGAAGTGCTTGCGGATTATCAACAAAGGCCTCAAAAACTTGTTGGTTTTTTCATCAAGAAACATCTGAACAAAGCTTG
AACCTGGAATGAAGATCAAAAGTTCTGTGTTACTTGCAGACTAGTAAATTTGTGTTATTGTTCATTCTGCTCTGTGAAAGTCCTGTCTGTTTAAATTTTA
GGAATTGATATCTTATATAATTTATAGGTCCCCCTGCTAAAGACTGTAAAACCTACAGCCCCTGAAATCAAGCACCTGCTTTCTTTATTAACCTGTGTGC
CTATCAATATGCAATTTACATAGCAGCCAGCTTAGCTGGGGGCAAAATGGTAAAAATATGTATATAATATAACATACATTTAAATTAAAATAATATTTTT
GCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF784650 [+]taattaatcccccccccccccattctagtggcagccgaccgtctggccctgtactggtgtgttgggcagtgagagcctggaacgtaactgggaacacaaa
caggacagaagagccagcaaggagcaagtgggagggaagtcggacaggtaacaagggtgagctgctctggaagaaggagatccaaaatggcacctgtgca
tactgctaagaaaacagaaaatgatcaagccacatcaaatcttgtccacgttgataatccacacctagagaaaatgaaagaagacattttgtaccatttt
gacattggaactgggactcatgattttcctgctatgtttggcgatgtgaagtttgtctgtgttggaggaagcccatccaggatgaaagcatttgcacagt
acataaatggagagttggcattgggtgaccccaactctgatgttgccaatatctgtgcaggaactgatcgctatgcaatgtataaagttggacctgtctt
atctatcagtcatggcatgggaattccatcaatatctattatgctccatgaattgataaagctgctgtatcattccaaatgcaccaacgtcacgattatt
cgcattggcacttgtggtggtataggtttggaacctggttctgtggtaataacaagtcagtctgtggatgcttcatttagacctcagtttgaacagatta
tccttggaaaaactgtagttcggaacacagagttggattctgagcttgcccgagaatt
3' EST: CF784649 [+]tttttttttttttttttttttttttttttttttttaaggcaaaaatattattttaatttaaatgtatgttatattatatacatatttttaccattttgcc
cccagctaagctggctgctatgtaaattgcatattgataggcacacaggttaataaaaaaagcaggtgcttgatttcaggggctgtaggttttacagtct
ttagcagggggaccttaaattatataaatatcattccaaatttaaacagacggactttccagagcagaatnacaataacacaaatttactagtctgcaag
taacacagaacttttgatcttcattccangttcaagcttttgtcagatgttttctgatgaaaaaacaacaagtttttgagggcttttgntgataatccgc
aagcacttcatgtgagctactgatctggtcccccttccaaacgggttcaaaagttgtaaaacaatacttacgggaggcttttcaattccacttttaaatt
ggcacattcccttgcaaaaaacaaaaaaaaaccccttttcaaaaaatttctttaacccccgggaaaaaaaaagccgggctttcaaaaaaagggcattttt
tttcttcccccccggaaaattaaaaaaaaaaaagggcccccatcaaaggggaaaaattttggaaccttttataaaaaaaaccccaagggggggccactgg
tgtgatttttccccaaagagaggggtgtgttaaaaaacccccggtgaaaaccccttttttgtgaacaaaccaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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