XB-CLONE-4351813
Clone Name: IMAGE:7027160
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Gene:
slc25a5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784654 [+]
taattaatcccccccccccccccccagccctaggaagcttttggtgctgcttcttgagcctagcatctgctaacgtaacaccggcatcaccatgactgac
gcagccatctcttttgccaaggacttcttggccggcggtgtggccgcagctatctccaagaccgctgtagcacctattgaaagagtcaagcttctactgc
aagtccaacatgcaagcaaacagatcaccgcagacaagcactacaagggcatcatggactgtgttgtcagaatccccaaagaacagggtttcatgtcttt
ttggcgtggtaaccttgccaacgtgatccgttatttcccaacccaggccctcaacttcgccttcaaagacaagtacaagaagatcttcctggacaatgta
gacaagaagacccagttctggcgctactttgctggcaaccttgcttctggtggtgctgctggggcaacctccctctgctttgtctacccccttgactttg
cccgtacccgtctagcagctgatgtgggcaaaggcaatgctgagagagagttcaagggccttggtgattgcttagttaagatctccagatctgatgggat
caagggcttgtaccagggattcaacgtatctgttcagggcatcatcatctacagagctgcttattttggcatctatgatacagctaaaggtatgcttcca
gatcccaagaaccacacattatcatcagctggatgattgctcaaactgtaacagcggtagctggatttgtctcctatccctttgaacacagtacgtcgct
cgtatgaa
3' EST: CF784653 [+]tttttttttttttttatttgaacctgccaagattgctgtggaagttctgcagagaatgctcaaacctcattaatgtaaatgctcaaaaacttcaaccaaa
ttgctgtgcaaaatacaatgtctactgatctgtttttatccagagcagctggattttaagtataatgttcaataccatagggcaggggggctttatttgg
tagtaaatctgttttttttattcaaactaaatttaccaccaggtcatgaattcaaaaataactagctggttttggggcactgagagcaacacccaagggg
ttggtgagcaacatgttgctcacaagccactggttggatcactgccataggggatgttatgatccctgacaagttgcactacagttcaagacttcagcta
caattatcttgcttggcttgaagggaagatacatccttaggacattattgaaggttaaaataagataaccatggaatgctctgaacccatagcctttttg
tgccttgatcacatgagattttagtggaagtgacaactgtaatttaccaagcgatgtcttccttattagaggaaggatcaacaaagggcaggagggtttt
accatgacatatggtgactaaatagcaacacacagaacatgagcattcctgcaacattgaccagccactaacagcctatcatttttgctttctttcagct
ctgtataatacatactcagttgggtatacaagccatagagaaaccccacaatcccctctagcttctaaaaatgcatgttcttaagggaattattagttta
acaatctgaacaaggttctggaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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