XB-CLONE-4351805
Clone Name: IMAGE:7027157
|
Gene:
depdc7
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC080467 [+]
CCAGTGCAGCAGTAGGAGAGCGGAATGGCCACAGTGCGGGAGAAAGCGGTGTCTTTGAATCTATGTGCGACTTACAGCCCCGCACAGCGACCCCCAGGTC
CAAATGGAAGCCATAGACCATTCCGAGCCACCTATATCTGGAGCAGCATCATCCAAGCTCTCACACAGGTAGAAGTGAAGAAGAGGCGGCACCACCTGAA
GTACCACCATGACTGCTTTATCGGCTCTGAGGCCGTGGACGTTGTGTTTGCCCACCTGGTGCAGCACAAGTACTTTGGGGATACGGAGATTCCCCGCTCC
AAGGTTGTGCGAGTGTGCCAGGCTCTCATGGACTGCAAAGTGTTTGAACCCATCGTGACGGCCTGCATGTTTGGGAGGGAGAAGAAGAGGACGGTGTTCG
AGGATAGTAGCTGCAGCCTGTACCGATTTATAAACAATTCAAGCCATCTTGGTGCCCAGGTGGAGAAAAGCAATGGACGTTGGACCCCTCAGAGACCCAA
ACACTCAAGCTTCCAATCTGCGCCACTTAAGTCTCCAAGCCTGGAAGATCTGTGGGATAACCTCAGTCTGACAGCTGCAGACCCAACGCACATAAACCTC
ACAGCTAACCTACCTCCCAAAGTTGTCGGTGAAGTTTGGCAAGAACAGACCATCCGCCGACTTCTGCAGCTTGTAGACCTACCTCTGCTCGATTCCTTGT
TGGAGTACACTCCTGTTGCTCCCAGAATTCCACAAGTGAAGGAAGAGGAGCTAAACCTCACCAGTAACTACCTGGACCGGGAGATCCTTAAAGCCTTCAG
TGATGCTCAGGCAGATGAGTGGGTCTCAGCCGCGGTGGATTGCTTGGATTTTCTGCCGGACCACATGGTGGTAGACGTGAGCCGGAACCTCCCAGAGCAG
CAGGCGCCAGACAGTAAATGGAAGCTGCTCCTGTTTGATACTATCGGCAAACACTACCAGAACCGGTCCCCGCTGCTCAGAAACCAGCTGTTCGATATTC
ATACTGGCATCGCCGAGCTGCTTGTGAATGGAAAGAATGAACCCGCTCTGGAGGCGACCCAGCTCTGCCTGAAGCTGTTGGACTCCCCGAGCCGCGAGGA
GTTCAGACGCCTCCTGTATTTTATGGCTCTTGCGGCAGATCCTTCAGAGTTCCGACTGCACGAAGAGACTGACAACAGGATGACTGTGAAAAGAATGTTC
TCTAGAGCCGTCGTCTGCAATAAGAACCTGTCCAAGGGGAAGTGCGACCTGCTGGTATTATTCATACTGGACCATCACAAGGATGTGTTTAAGATACCCG
GCTCCTTGCACAAGATGGTCAGCGATAAACTGGTAGCAATTCAGCAAGGCAGCGATCCAGACCGAGACACAGGTTATACTTTCTGCCAAAGGGTGGACAA
ACGGGAATTTGATTCAGCCACGCAGAACAACACTCGGACGGAACTCTGCGCTTTGCTGAAGACCATTTACGAGAACACCAGCCTCTCCCCCAAAGAGAAG
AAACGGCTCCTGGGCCAGTTCTACAAGAGCCACCCAGAGACTTTTATCCAGTACTTCGGAGACCGCGTGAGCAGCGTCTACACGTAGCGACCTCGGCCCA
TCTCTGTGTATAGATCTGTACAATGTCTTTGTAAATATGAATTTGTTAATATATTGAATAACTAATTGTTTTTGCACATTTAATAAACTTTCAAATATTA
AAAAAAAAAAAAAAA
5' EST: CF784659 [+]taattaatcccccccccccccccagtgcagcagtaggagagcggaatggccacagtgcgggagaaagcggtgtctttgaatctatgtgcgacttacagcc
ccgcacagcgacccccaggtccaaatggaagccatagaccattccgagccacctatatctggagcagcatcatccaagctctcacacaggtagaagtgaa
gaagaggcggcaccacctgaagtaccaccatgactgctttatcggctctgaggccgtggacgttgtgtttgcccacctggtgcagcacaagtactttggg
gatacggaaatccccctctccacggttgtgcgagtgcgccacgctctcatggactgcaaattgtttgaacccatcgcgacagcctgcttgccttggtagc
gacaagaatacgaccgcgttccacgatacctatctgccttcctgtacccgatttacaaacccattccaaaccaaccaatgcgtgccccattttggtacaa
ataccccatgtgacgtttggaaccgccttaagaacaccccataaacatctcaaagtcttttcaacaatctgtctgctaccccttcatgtttctttttacg
cccccctgggacattattcattgttgggagaattaatccctttcacggttctaggataaatacctttgacctcatatatctattccgcgctatttgttta
ttacccctcccccgcgttttataattttaccactctcttttataacccatagaccacgccttttaccggatcttggcactacgataaacctataaccctc
gtccgggtgtgtaacctatcttggccgcgtcctttttgtatgtactattaacccacccggagacctcgaaacccccctttgtttcgcgtatattacccac
tctctctgatttctctctatccccctaaatttttctaacccatatttgcccttgtgtaattacgccccctctattatttctcttacttctttgctccccc
actccatttacacaaattgggctgacataaccccactattn
3' EST: CF784658 [+]tttttttttttttttaatatttgaaagtttattaaatgtgcaaaaacaattagttattcaatatattaacaaattcatatttacaaagacattgtacaga
tctatacacagagatgggccgaggtcgctacgtgtagacgctgctcacgcggtctccgaagtactggataaaagtctctgggtggctcttgtagaactgg
cccaggagccgtttcttctctttgggggagaggctggtgttctcgtaaatggtcttcagcaaagcgcagagttccgtccgagtgttgttctgcgtggctg
aatcaaattcccgtttgtccaccctttggcagaaagtataacctgtgtctcggtctggatcgctgccttgctgaattgctaccagtttatcgctgaccat
cttgtgcaaggagccgggtatcttaaacacatccttgtgatggtccagtatgaataataccagcaggtcgcacttccccttggacaggttcttattgcag
acgacggctctagagaacattcttttcacagtcatcctgttgtcagtctcttcgtgcagtcggaactctgaaggatctgccgcaagagccataaaataca
ggaggcgtctgaactcctcgcggctcggggagtccacagcttcaggcagagctgggtcgcctcccgagcgggttcattcctttcattcacaagcaactcg
gcgatgccagtatggatatcgaacagctgggtttctgagcagcggggaccgggttcttggtagtggtttgcccgatagtatcaaacagggaccagctttc
attttaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: