XB-CLONE-4351799
Clone Name: IMAGE:7027155
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Gene:
rplp0
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784663 [+]
aattaatcccccccccccccgcttttcaacttcccggagccctagtgtgagggaatatctagtctttaaacccttttgtggcaatccttggaagcactga
caagatgcccagggaagacagggctacctggaagcccaactatctcctgaaaatcattcaattgctggatgattatccaaaatgcctcattgtgggggcg
gacaatgctggttcaaaacaaatgctgcaaatccgtatgtccctgcgtgaaaaagccgtggtgcttacgggaaagaatatttgatgcgcaaggttattcg
tgggcctccggaacacctttcccctctcgagaaatttccgtcctcccaccaagggtccacgcgtgtttttgctttcatttccgaagatcttttcgacgtt
ttgccacctcgtttttcggcccccttgcttttgccctgctgcctttcgctgcgcccctttccccctctttttccggggtcttatattgcctcgtcctttt
tatttaccgtgtgtgccgcggtttctcactataattaacatttcattttcacatcccatggatatattgagggcgtattttctctcccgcgagtattttt
cttttttcaccgtctacagattttttctttacccgcgacaacatccctccttgtgactcttgcaattgtgaaggcctacactcctctttcggctcgcacg
ctgtaatgagtgggtgtcgtatgcgacagacaatttgttgtggtcgcggacgcctattatgtttggtgagtgcgccccattcctcacgttatgtctcgta
agatatatacacgacgtattatgattgttgacatcacactcacaccccccccgcccttttttactccgcgcgctcatgagatagtgcgattatctatgct
ctactatacacttcgcgtcatgtcagtggttattacccacagntatatcnacattctcgctaccgtacatatttaattgatctattagtagcacttngca
gtcaacatagtacggttattacgcatacgcgcaaagatatacacacacccatatgtattacgcctcacataaacgncgttatactctacatattattcta
cgctcgagcttaatattttatgaacagagctgtaacgctctgtatattttataaccaccgaccgctatatatttattgtgtatacaacagtgtgcacgcc
ccctctctctgtttttacttatttgtatcctccgccctctcttttcttattt
3' EST: CF784662 [+]tttttttttttttttcttttagagactgtaaaagtctttaattatgaacttggaacaatcaaggtttagtcaaagagaccaaatcccatatcatcatcag
attcctcagactcttcttgcttttcctccttagcaggagcagcagcaggagcagcagccacgtcagcagcgggagcagcaacaacaaaagctgatggatc
tgccaggaatgccttgaccttgtcggccagggggaagctgtagtctgtttcaactgcaatggcaaggacacgcttgtatccattgatgactgagtgaggc
acagaggccacggtgggataaccaatctgaagacagacactggcaacattacgaacaccctcaaggaagcgaacatgcaaggcctcctcagtgatatcca
gcacctctggactatagatgctgccattgtcatacacttgttgaatgattaatccataagaaaatggggaaatattcaacatgtttaagagtgtggcttc
actggcacccactttatctccagtcttgatcagctgcacatcactcaagatttcaatggttcctctggagatttttgtggtgatccccaaagcctggaag
aaggaggtcttttcagggcccaatccagtattctgagcgggcacaacgaccccgcaaggtgctattgctcctgcacgggcacagggcaggaacctttatt
tgccagcaacatggtctctggacctcagtaagatctttccttttgtgaaaaacaaaggccccacattaccccttgatggtgaagaaaggaagttttct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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