XB-CLONE-4351793
Clone Name: IMAGE:7027153
|
Gene:
serbp1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784667 [+]
taattaatcccccccccccccgagacagcctctttcccagccatcttttggcttactagcaagcgagctctaggctccaggcctcgtagaatcggaggaa
catccacctcttaaggggccatcatgcccggtcacttgcaggaaggcttcggctgcgtcgtaaccaatcggttcgaccagctatttgacgatgaatctga
ccccttcgaggtgttgaaggcggccgagaacaagaagaaggagggcgcagggggaccgggtcaagggaccggcaaaacggcagctcaggccgccaaacag
cccaagaaggagtcccagaaagaccggaagaaccctctgtccgaaaaggaggagaagagccaggcgcctgtaccaatcaagaaggaagctatcaggcggg
ttggtcggagacctgatcagcagcagcagccgccgcagcagccaccaacacaacagcagcagcaacaacaacaacaacaacagcagcagcagcaaccacc
tcagtcatcacagggtgaaggaaagccagttgacaggaggcagtccgataggcggccaccccgtgaacgccgttttgaaaaaccaactgaagaaaaggga
gaagcaggggaaattttctgttgaccgacccatcggtggataggcccatccgttgggtcgcggtggccctgggtggaaaggggtggcccgtgtgcggttc
gtgggacgtgggttttgggcaaggggccgaatggcttttgaattctcggtggaaaaaacgtgaaattttgacaagggcatagagggggcgagggaacaga
aaacctttctttcacaatttgttcaggtgggggcccctaaaggtccttggaagggaa
3' EST: CF784666 [+]taagcatgcaagcctcgtagaaggggtccctcaggggcaccaaagttggacaagtcatccaagcttacgccaaagctggaaaagcttcggggtcatctac
atcaggagcagaagcatttgacttgtcagttcttcctccacgactaggcctaccgccgcgggcacggccgcctcttcctcccctgcctccacgaccaggg
cgtccaagatctccaaagttaatttcaagttgggaagttatgtcatttgcaggcttacggaaatgatgatccagggcatctgactcagcatgaacctcat
cactctttgctttatgcaggacaaagcctttcttccattggccatcagcaccttcatttggtttacggatgttgaattcaacttttgaacgttctttgtc
ttgcattgctttccattcatccagagtcatttccttaggaccttcttccttcacttcttcagcctcattttccttgttttcagaatcaacagcaggatgt
tcttcagtttcaggagtttcctcagtgactgcagactggtccagttcacttaattcatccttaacagtgccccagttgtgtgatccacttcctccccgtt
tatcctcatgctttgggccactgaaatgtgaagaagctctgtcactgccactatgcctgtcaaattcacgttttccacgagagtcaaagccatcgcccct
gcccaaaccacgtccacgaacgccacggccacccctttcaccagggccaccgcgaccacggatgggcctatccacgatgggtcggtcaacagaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: