XB-CLONE-4351788
Clone Name: IMAGE:7027151
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Gene:
ppp1r8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784670 [+]
taattaaccccccccccccgagaggcttcccataatgcttagggaggaggactaaagatggcggcgaatccgaactcaagcaaacctatatttgagtgcc
cgagctgggcagggaagccaccccagggtctgcaccttgatgttgtgaaaggagacaagctcattgagaaactaattattgatgaaaagaaatactacct
atttggtaggaacccagatatttgtgactttacaattgatcatcagtcatgctccagagttcattcagccctggtgtaccacaagcatctgaagagggtc
tttctgatagacctcaacagcacacatgggacctttctgggccatatccgattggagccccacaaacctcaacagatccctatagattccacaatttcgt
ttggagcatcgacacgcatgtacacgttacgagaaaaacctcaaacacttccatctgccttaaaaggagacgaaaaggcaggggaagatgatgaattaaa
aggcctcctgggccttccagaagaaagagactgagcttgataatctcacagagttcaatactgcccataacaagagaatatccacgttaactatagaggg
aggcaatattggatattcagagaccaaagaggaagaagaaaaatttcccgtgtaaactttcatggaggaatgaggaggtttattaatcccccaaggaatg
gttgatcctttcgggttggggcgttttcacaaaataatggggggccaaaacaggctggtggggttcccgtggtaaagnaaaaaatgaaaaaccccaaaaa
ttttcctctgggttccccctccaggttcttaaaaaaaaaattcaagttgc
3' EST: CF784669 [+]atttctgtgctgcaatatatgcagtttactagcaaactgaaaagcacagttctctttctgaaggttttttcatcctagatacagcaccaggcacataatg
agggaaccacagatctccatatagtgcatgcacgaaacctattgggagggggtgcacttgcaatcgctgctccctcagtagttacaccactatacagcaa
tgcaccttcttttctacatggggagaaccaatcaaagtagtgttaagaaggaattacaccattaacattagactgatgttaaatacaaaaactttactac
ttctgctacctgattatagagcaacatcctcttgtttttagctactggtgaattctactgtctgcttaatgagtattctggttttaaataaacattctcc
tctgcattcgaatatggaataggtggaggattaggtactgaatttatgagatacactatcttcttgctggtaaatgtaaccctgactggcggccactgca
tagtaacatgggcaaataaaaagaaaattaatgtgagttagatttcacccatttacacacacacacacacacagtcaaataaaacaaagcaagaggaaaa
gtttacataaatgtatagaattcacatattacatataaaaatatctctaaagctgtacagttgcatgcagatttctatacactgtatataggtggccaac
ataaaacacattgattctattcccctttgtcagtaatatatttttcccatcactgctgcctctatgtgactgatatattgatttagcgtgcatgaactcg
tct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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