XB-CLONE-4351772
Clone Name: IMAGE:7027144
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Gene:
tomm5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784680 [+]
taattaatcccccccccccccattcctatacctgcatctgatcgctcttgtgcttttccatcacacgatgttccgtctggaaacccttgggtcgaagtgt
gatccagaggagctgaggaaaaagatgcggcaagacgtgctgacctcagttcgaaattttcttatatacatagcagttctgagaatcactccatttgtct
tgaagaaactggacagtatatgaaacaatgaactgaagagctccacaaatgaagcttattgaagagtgggacctgcctggacaacaacaaattatggata
gtgttttacaaaaagtgaacttaatatatttttcacatccattagactgaggtttttccagcttttgttttgcctgtgactgtttgagaactgaccattc
tttttaagtaaaatgtcactggcttttgtcaaaaaagaaacttaaaagtgtaccatgggcctgttatgccagaatgaacctaatagttaaagtttttttt
ttccccgtataaaaaaataaaaaaaaagggatctcccctaatttctcaaaaggcggtccgccccccctccccggtgggaaaacttaagagtcccgagggg
acccacaacctgtgtgaattaattatgcccagtccttctagaaaagcaggcggtttagcacagaactggtcgaaaagacatcaacaattgaagttgtttc
ctacatacatatatatgttttcgctacatccgtgaaagacatataacaatgtcccaaaagtttagttgtagatatctcctcgcctcgagcgctaatatct
cactaaatatgattgtgaggggggagagatttgggtcacccccagaaaacattatcaaatatcaaaaagggtggcgatctttctttttaaaaccgactgg
taatgtgttgggggaaacaccgggggatgaaaatatctctatttttgcgttgcgtacgccgtaattatacatatttataggcgccctatagaaaaaaata
taaccatcactcactaacataagcaagaggaaaaccatatccgggtttattgaagggcagtatcctcatatagtgtagaagagcgtcgtggaaaaaaaga
taatcggcagcgcactatgttgggcgcaacacggccgacatccttttaatgtttaagg
3' EST: CF784679 [+]tttttttttttttttctgtgaaaaaaaaactttactacttagttcatcttacataacagcccatgtacacttttaagtttcttttttgacaaaagccagt
aacattctacataaaaagaatggtcagttctcaaacagtcacaggcaaaacaaaagctggaaaaacctcagtctaatggatgtgaaaaatatattaagtt
cactttttgtaaaacactatccataatttgttgttgtccaggcaggtcccactcttcaataagcttcatttgtggagctcttcagttcattgtttcatat
actgtccagtttcttcaagacaaatggagtgattctcagaactgctatgtatataagaaaatttcgaactgaggtcagcacgtcttgccgcatctttttc
ctcagctcctctggatcacacttcgacccaagggtttccagacggaacatcgtgtgatggaaaagcacaagagcgatcagatgcaggtataggaatgggg
ggnggggggggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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