XB-CLONE-4351766
Clone Name: IMAGE:7027142
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Gene:
fam20b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784684 [+]
taattaatcccccccccccccgcatttcgattgggtgctaagctttgggcagttttatgtgacccaataagcttcgcaaggaatgtgccgatgtacccga
ttggttcccggtggctaatgcaaccgaagcggattggctatcttgacagttccgatgtgcggcgcactgtgtgcttgtggaggtagagtgtgtgatgttg
gcaactggggatcagcaaccgtgtgttggaggttcctgcaggaatcaaacagcgcaatgccctgcacctggcacacataggacatcacccttctttgcta
agagctttttatcttgggcatcatgaagctaaagcagcgggtcgctgtcttggctctgctgctggtgattttggttctcaccaagcttcttttattggac
cgcctggagacctctgctgctcagaggcaggaccagctgtcttttgagcgtatgatgtccggcttacggctcaccatggactcccgtttggagcataccc
tgcagtccccttgggagattgcatcccaatgggtcgtgccacgggaagtgtatcctgaggatagcccagaaatgggagcagtattgcattctatggcaac
caaaaaaattgtgaaagctgatgttggctacaaaggcacacagcttaaagctttgcttgtcctggaaggtggacaaaagggtggtatttaaagcccaaaa
agtaccagcccgtgaattatttttgtgggaaggaaaaacccctacgcttgggttatgaacagggcattaatggcctgaaaaatcaaactgggcttttttc
attttaagaaccaggaaat
3' EST: CF784683 [+]tttttttttttttttatttttttttttttctaataaatatatcgatctcgtaaaaacattattaaccagaattctgcatacattttagattgatattact
tgtaaaataaaagataaccagtgcctaattaaaaagaaaaaaggaaacttaaccataattgtagccaaaaacccagatgagtttgccttgcttatgcagc
tctccatattctgtcagtggatgctgggagctttaattcaacagcaggaggctgcaaacctaacagtcctaacatccacttgcctcactataccattatc
ccaaagcttcatctcctttctagaaaataaatgtatctgtggaggggcagttgagactggttacttttcagctgctttgacattaccccatacatgtcac
cagtcattttgccaggagggcacctagatgcaaatatgaattcttttgacgaaggctaaaaaaatattggcaccactgatctaatgaggttgccaaaaca
ttctctctggcaaatgcccttttctaccttttcattccgaagtttcttaggttggggcactgaaagagccctgtatatatatatatacgtgcacgcaccc
tattcttaataatgggggacctttcccaggattgataaaaacatacggggtgacccttgggaaatctcgaggctgtgggtctctgacactgaagggctat
ataagtatatggagaaagaagattttgtggcttaatatgccagcagaaaagcaatgtatcgttttgcatgttaacaaactacgtttcaaaaagagtcagt
agttttagtcagttcccaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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