XB-CLONE-4351744
Clone Name: IMAGE:7027133
|
Gene:
cs
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC080507
[+]
GCTTTCAGGCGCCTTCGGCTCTGCCGGTTCCTCTAGGAGACAGCGCTCAGCTGCGGCCGCTCTTATCAGACCTTTCATTCGCCATACGGAGATCCTCGTT
GTAGAGTCATGTCTCTCATTACAGCTGGACGCCTGTGCGCCCGTATCCTCGGAGCCAAGAACTCCCCTTGTGCTCTGATTGCTGCCCGGCAGGCTAGCTC
TTCTGCGAATCTAAAAGATGTCCTGTCAGATTTAATCCCCAAAGAACAAACTAGAATTAAGAATTTTCGGCAGCAGTATGGCAAGAACGTGATAGGACAG
ATCACTGTTGACGTGATGTACGGTGGTATGCGAGGGATGAAGGGTTTGGTTTATGAAACATCTGTGCTTGACCCTGATGAGGGTATCCGTTTCCGAGGCT
ACAGCATTCCAGAGTGTCAGAAGTTGCTCCCCAAGGCTCCAGGTGGAGAAGAGCCATTACCTGAGGGACTCTTCTGGCTTTTGGTGACCGGGGAGGCACC
TAGTCAAGAACAGGTGAACTGGATTTCCAAGGAATGGGCAAAGAGGGCTGCTCTTCCTTCCCATGTTGTGACCATGCTGGACAACTTCCCTACCAACCTG
CACCCCATGTCACAGCTCAGTGCTGCCATCACAGCATTGAACAGTGAGAGTAATTTTGCTCGGGGCTATGCTGAAGGGGTCAACAAAACCAAGTACTGGG
AGTTAATTTATGAAGATTCCATGGATCTGATTGCCAAGCTGCCTTGTGTTGCCGCAAAGATCTACCGCAATCTTTACCGTGAGGGGAGTAGCATTGGCGC
CATTGATTCCAATCTAGACTGGTCTCACAACTTTACTAACATGCTGGGTTACACAGACCCACAGTTCACAGAGCTCATGAGACTTTACCTGACTATCCAT
AGTGACCATGAAGGAGGCAATGTCAGTGCACATACAAGCCACTTGGTGGGTAGTGCATTGTCTGATCCTTACCTTTCATTTTCTGCGGCCATGAACGGAT
TGGCAGGACCTCTTCACGGCCTGGCTAACCAGGAAGTTCTTGTGTGGCTCACAAGCCTGCAGAAGGGTCTTGGTGGAGAGGTGTCTGATGAGAAGCTGAG
AGATTACATCTGGAACACGCTGAACTCTGGCAGGGTTGTCCCTGGATATGGTCACGCTGTGTTGAGAAAAACCGACCCGCGCTACACGTGCCAGAGAGAA
TTTGCACTTAAACATCTTCCAAATGACCCTATGTTCAAACTGGTGGCTCAGCTTTACAAGATTGTTCCCAATGTACTGTTGGAACAAGGCAAAGCCAAGA
ATCCATGGCCTAATGTGGATGCTCATAGTGGGGTCCTCCTGCAGTATTACGGCATGAAGGAGATGAACTACTACACAGTTCTCTTTGGCGTATCGCGGGC
ACTTGGTGTTCTGGCTCAGCTTATCTGGAGCCGAGCTCTTGGATTCCCCCTGGAAAGACCAAAATCAATGAGCACAGATGGCCTCATGCAGCTGGTCGGA
TCAAAGTCTGGCTAATCAAAACGGGAATCGCCAGGGCCCCTCCATTTTGTATAGAGGGCTCTTTAAAAAAAGACAGTACACCTTTTGTTTCAACGGACTG
CTTTGCACCCCACTGTTTTCTGAATTCCGATGAGAAAACATGTCTGGTTATTTATGCTGAAAATCCATCTTGCAGCTCAGTCTCAGCCCCTTTTCTTGCT
GAAGAGATCTGTATTCAGTTCTCCATCAGGAAATGGGATCTTGTGCTGCTGGTTAGAATCAGAACTCTGGAACCCCCTCAGTCACAATCTGTTAACTCAC
AGACCCCATCATTCTGCTTATTTATAATTCAGCCACGGGCAGTCAGTGCCTCCTAATTTTGTTACTAATCTGCAGAAGCAGTGGCCCATAGGTTTTCATA
CACAGGCTTGTATGGCACAGCAGCCCCCTTTATATGTCCCATTGTGAAACGCAAAACCTGCTATAGACTTGCCTGTGCTTAGCACAACACTGCGCCTGGC
TTCATATCTTCAGGAGCATGGGTTTCATTTTCTTACTGGGAAATGTGATTATGTTTCTGCAACCATTTAACTGCTGTAGGTTGGCTGAAGGGCCTGCAGG
GCAGGAGAAGATTAAAGTCAGCAGTATTGGACTTTGGAGGCCATCAGAATATACTGTCAGGCAAGGGTTTGGCATTGTATCATCCCCACTTGTTCTTATC
CCATGGATGCTCCTCATCTATCACTTTTTGTTTTTAACATGTAGGTTTTTATGTAGGTGGACAGATAACCCTGCCTGCTGACCACAGTGAAATTATAAGG
GAGTAGAAATGTCCATGAGCAAGAATTATTGTGGATGGGTTGCTGCTTTTAATGTCCCCCTACTAAAGTAAGGCTCTACTGTCGTTATGCTTTTATTTGC
AGCTCTTTTTATGTGTTCCAGCACTCTGGATCCTCCTGTGCTGTACTGGGCCTTAAAATATTGCTAACGACCTTTTAGTGCTTTACAAACACAACTGTGA
ATTCAAAGGATTCAGTTAGAGAGAGTTTCTACTGGTTTGTTTTTTTTTTTTTGTTTTTTTTTTTAAAGCTACCTTTACTTTAGCCCCTTCCTTGCTTGTG
GCAGCAGTCTAGCAATTCTTGCCGCAGTGATCAGCTCCCATCATCCCAAGGACTACAAAAGCCTGGGAGCCTTAAGCATTGCTACCCTATGGGGTCTTGT
TTGATTACAGGGTTCTGCTATCCAAAGGCCACAGTTGGACTGAATAAATGCTCATTATACAGTGTCGGTGTGCTTGTTAGTGTGCATTTCCCACTCCTTC
TGCACTAGTTCATTAATCCTCAGAGATCTGCAAGTGCACTTTCCCAAGCTGCTTAATTTGTCTTCCTCTTGTGCAGGAATGTCAGGCTGCCAGCCCTTTT
GCAAAGTCTAGTTAGTGATATTAGGACTCATAATTCCCAGCTGTTGGGGGTTACAGTTTGGCATCTCTGCTCTACAGCATGACTGTCCACCTAGAGACCC
AAAAAGGGATCCTGTGACCCATTTGTTAATACACTCCTGTCCACCTGTCAGCCTTTTCCCATGTGGTATTGCCTAACCTGTTCTTGTCAGTGCAGACTAA
TTGGCCATCAAATGCTCTGCAAATTATGGATTGATATCCCAACTTACCAACAGACTTAAGGTTGTTCTGTATAAATGCAAAATAAATGTTTTTATTACCT
ACAAAAAAAAAAAAAAAA
5' EST: CF784698
[+]taattaatccccccccccccgctttcaggcgccttcggctctgccggttcctctaggagacagcgctcagctgcggccgctcttatcagacctttcattc
gccatacggagatcctcgttgtagagtcatgtctctcattacagctggacgcctgtgcgcccgtatcctcggagccaagaactccccttgtgctctgatt
gctgcccggcaggctagctcttctgcgaatctaaaagatgtcctgtcagatttaatccccaaagaacaaactagaattaagaattttcggcagcagtatg
gcaagaacgtgataggacagatcactgttgacgtgatgtacggtggtatgcgagggatgaagggtttggtttatgaaacatctgtgcttgaccctgatga
gggtatccgtttccgaggctacagcattccagagtgtcagaagttgctccccaaggctccaggtggagaagagccattacctgagggactcttctggctt
ttggtgaccggggaggcacctagtcaagaacaggtgaactggatttcaaaggaatgggcaaagagggctgctcttccttcccatgttgtgaccatgctgg
acaacttccctaccaacctgcaccccatgtcacagctcagtgctgccatcacagcattggaacagggagagtaatttttgctcggggcctatgcttgaag
ggggtcaacaaaaccaagtactgggggagtttaattttatgaagaatttccttgggatctggattggccaaagcttgcccttgggtggtttgcccgcaaa
gaattcttacccggaaattcttttacacggggggaagggggga
3' EST: CF784697
[+]tttttttttttttttgtaggtaataaaaacatttattttgcatttatacagaacaaccttaagtctgttggtaagttgggatatcaatccataatttgca
gagcatttgatggccaattagtctgcactgacaagaacaggttaggcaataccacatgggaaaaggctgacaggtggacaggagtgtattaacaaatggg
tcacaggatccctttttgggtctctaggtggacagtcatgctgtagagcagagatgccaaactgtaacccccaacagctgggaattatgagtcctaatat
cactaactagactttgcaaaagggctggcagcctgacattcctgcacaagaggaagacaaattaagcagcttgggaaagtgcacttgcagatctctgagg
attaatgaactagtgcagaaggagtgggaaatgcacactaacaagcacaccgacactgtataatgagcatttattcagtccaactgtggcctttggatag
cagaaccctgtaatcaaacaagaccccatagggtagcaatgcttaaggctcccaggcttttgtagtccttgggatgatgggagctgatcactgcggcaag
aattgctagactgctgccacaagcaaggaaggggctaaagtaaaggtagctttaanaaaaaaaacaaaaaaaaaaaaacaaacccgtagaaactctctct
aactgaatcctttgaattcacagnttgtgtttgtaaagcactaaaaggtcgttagcaatattttaaggcccagtacagcaccagaagatccagagtgctg
gaacacantaaaagagcctgcaaataaagcatacn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: