XB-CLONE-4351724
Clone Name: IMAGE:7027126
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Gene:
tmem111
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784711 [+]
taattaatcccccccccccgggatttccgtttccgctggctgactggctgaggaacatggggagccccgagttgctgttggactccagtatccgcctgtg
ggtcgtcttacccatcgtctgtatcaccttcctcaccggcctcctccgccaccatgttacccgcctgctgcagggggagaagcgccccgaactggagccc
ctcagcgatagccaggtgctgatccggagccgcattcttagggagaacgggaaatacatcccccgacagtcgttcctcatgaggaagttctatttcaatg
accaagagacgggatttttcaagaaaaccaagaggaaagtgatccagcggaacccaatgaccgaccccactatgatgaccgacatgatgaaggggaacct
caccaacgttcttcccatgatcctgattggagggtggataaactgggctttctctggcttcctgaccactaaggttccgttccccttgacgctccgattc
aagcccatgttacagcgtggcatcgagctggagtcactggacgcatcatgggttagctccgcctcctggtatttcctcaatgtctttggcctgcgcagta
tgtacactctgctcttggggcaagataacgccgccgaccaaagccggatcatgcaggatcagatgacggngggccgccctctccgtgccgccggacacca
acaaggctttcaaggctgagtgggaagccatggagatctcg
3' EST: CF784710 [+]gttctactgagccccggcaagttccagatcctgagcgatcagatcttcctcgatggcctccagcgcccagtgatgttccgagatctccatggcttcccac
tcagccttgaaagccttgttggtgtccggcggcacggagagggcggcccccgtcatctgatcctgcatgatccggctttggtcggcggcgttatcttgcc
ccaagagcagagtgtacatactgcgcaggccaaagacattgaggaaataccaggaggcggagctaacccatgatgcgtccagtgactccagctcgatgcc
acgctgtaacatgggcttgaatcggagcgtcaaggggaacggaaccttagtggtcaggaagccagagaaagcccagtttatccaccctccaatcaggatc
atgggaagaacgttggtgaggttccccttcatcatgtcggtcatcatagtgnggtcggtcattgggttccgctggatcactttcctcttggttttcttng
aaaatcccgtctcttggtcattngaatagaacttcctcatgaggaacgactgtcgggggatgtatttccccgttcnntcctagaatgcggctccggatca
gcacctggctatcgctgaagggctccagttcggggcgctttctcccctgcagcaggcgggtaacatgntgncngaagaagcccngtgagaaggtgataca
gacgatgggtaagacgacccacaagcggatactgggagtccaacagcaactcgggggctccccatgttcctcagccagtcagccagcgaaaacggaaatc
ccgggggggggggggatttaattacttcca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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