XB-CLONE-4351715
Clone Name: IMAGE:7027123
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784717
[+]
taattaatcccccccccccccccgagtgtgagatctgagcatcactgtggcgctagggaatcccaggcagtgagaagggagtagcctcgcccagcaccat
caccacacagggagggcaagtacgggactagttctaacatctaaaaaccttggcttcttcaggtgaaagcactatagctggaggctcccaggcgccctat
ttttctaaatttaagcctcagacatgaccaagctgaacctgttgctggccctgtgggctgtgggatgtggcatgttatcaatggcacagagtactacagt
cactgtagcaacaatgccactgaaaactataactacaactatggacacggcaactacattaattacaaagcctgtaactactgctatatcaaccacttca
gttaaaagtctcagtacttctgtaaccagttcaacagcaaactctgtagcaagtgcctcaaccaccaacatcaccaatcctcctacaacttcattaccaa
catccagtacaccaaacaaaactacagagggaaaaatggattcaactatcactacatctgtgactccttcaccacagccttcattaaaaccaacagcaac
cagctctacagtaaatcctgtatcattttcaagtaaagcagaaactccccagactattggaacaacagtcaagcaaagaacttttcatctcccagcatta
gccaactttccaaatcttgccacaacctcttgctagaaccaactccccaggaccccaagaattacttatagccctaaccaaaaccacccgagcatccagg
cttccacctcctcttccccctgtttaaacacggtccccaaacccttttctttttt
3' EST: CF784716
[+]tttttttttttttttgaggtctaaatgtgtttaataggatatggggtagatgaaaattccagtatatgccctttgtactcagtgttcactatctgctgtt
cctcatcctctccattctgtatgtaaatacttgggcactgtatatgtgtactgatccgtttccttacaaagtactgtgcagttctcgcctccatgtagga
aagttctgttccagggttaagaataatcagtgctttttgagagcaaagtacagggcagttatggtgaataagagtgagctgcttctttgaatcttcctaa
acatcacaaaatcagatgtatcaaatgagcttatgcagtatgcaaagtaaatcggacagattacaagaaactgggactgaaattgtctggcttatatcag
tcaattcttatttgcttggccagttgtgtgaatatatatacatatatattgcactgctggataccccaaacagtcttattgattttctacagctttataa
aatgggtgtcctcttcttcttcaagctcctctcttaggttatccagtggaacaatccagctatccccaagctctccattaagacccccttttttctcttg
catctctggagctgtctccattacctctagagtgggattatcagggtatccattccccatggtttgaaattcctctgaaaacctcggttccccttttaga
aaactgcccctgatgccaaccatccataaacggctgccatggaaccccagggacaccgccccaactttacaatagtttttttttaacagggatgccttag
ccaaatctt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: