XB-CLONE-4351709
Clone Name: IMAGE:7027121
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Gene:
fth1.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784721
[+]
aattaattcccccccccccccccaaaacctcctctctggctctgagtagagttcttgcttcaacagtgtttgaacggaaccctctctgagtcttttttag
accaaaatctctctctctctcttgcattactcttttttttgtgacttttttgcgccaccagcaaacaccgaaacgccgccatgcaatcccaggtgcgtca
gaacttcaacagcgactgcgaagccgccatcaaccgtatggtgaacatggagttgtatgcctcctatgtctatctgtccatgtcttactatttcgatcgt
gatgacgtggcactccaccatgtggccaagttcttcaaggagcagagtcatgaggaaagggagcatgccgaaaagttcctcaaataccaaaacaaacgtg
ggggccgcgccgtccttcaggatatcaagaaaccagagcgtgacgaatggggtaacaccctggaagccatgcaggccgctctgcagctggagaagactgt
gaaccaggctttactggatctgcacaagctggcatctgacaagactgatcctcatctctgcgacttcctggaatctgagtacttggagggacaggtgaag
gccatgaaagagcttggagactacatcacaaacctgaaacgccttggggtgccgcaaaacggcatgggcgagtacctgtttgacaagcacacccttgggg
gagagtagcttagccgcacttctcagggtgggaaacaaacccagtcagctcctctgtttccaaaaaactggccttctttaacatatcggggggggaatta
acacccataagctccccatggcccccctggttggaacacctttt
3' EST: CF784720
[+]tttttttttttttttggaatgctgaaaaaactttattgaaaacatgttgacaagcacagcagcttctgcatgattcagaacagtaatagcacagatttat
tcccatcatcctcaagataaaaaggtgtcaacaggggcatgggagctatggtgtaatccacacagatagtaaagagacagtattttgaaacagagagctg
actggttgtttccaccctgagagtgcgcttagctactctcccccagggtgtgcttgtcaaacaggtactcgcccatgccgttctgcggcaccccaaggcg
cttcaggtttgtgatgtagtctccaagctccttcatggccttcacctgttcctccaagtactcagattccaggaagtcgcagagatgaggatcagtcttg
tcagatgccagcttgtgcagatccagtaaagcctggttcacagtcttctccagctgcagagcggcctgcatggcttccagggtgttaccccattcgtcac
gctctggtttcttgatatcctgaaggacggcgcggcccccacgtttgttttggtatttgaggaacttttcggcatgctccctttcctcatgactctgctc
cttgaagaacttggccacatggtggagtgccacgtcatcacgatcgaaatagtaagacatggacagatagacataggaggcatacaactccatgttcacc
atacggtttgatggcggcttcgcagtcgctggtgaagttctgacgcacctgggattgcatggcgggcgtttcggtgttttgctggtggcgcaaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: