XB-CLONE-4351704
Clone Name: IMAGE:7027119
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Gene:
akr1c3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784724
[+]
taattaatcccccccccccccacttgtcctatatacagtgtaacccccagggaggtgcagtaaggatctgatcgcttacaccaaccatggcactgcacaa
agactcctacgttgagctgaatgatgggcacaaaatgccagtgattggatttggcacctacgcccctccaaagttccccaagagtttggcagaggagggc
accaaagtggccattgacgttggataccgccatattgactgcgcttttctctatgggaatgaggaggaggttggacgggcgatcagggcaaagattgcgg
atggtaccgtgaagagggaggacgtgttctacactgggaagctctggtccacgtcccatacccccgagagggtccggccagccctggagaaatccctgaa
agaccttcagttggattacatggatctgttcatcatccatatgcccatggagttcaagcctggagatgatctctttccggcggatgaaaatgggaaattt
atttaccataacacggaccttagagatacatggaaggctctggaaaagtgcaaagacgcagggctggtcagatccatcggggtgtccaattttaaccacc
agcagctggagctgatcctcaatatgcctgggctgaaatataaacccgtctgtaaccaggttggaatgccacgtcctacctgaatcaaaaccaaattgct
ggaagttcttgcaagttccaagggacatccttggttggggggggaatacagccgttattggggggcccccccaggggaatgaaccgcaccggaaattaaa
ccccaagccctcccctggtggcttcccccgggaaagaaccctttggctta
3' EST: CF784723
[+]tttttttttttttttggagactaattcaatactttattgtctaatatggaggcaatggggagaggagggggcagtaacaaatgtggatcttctgctcatt
gcaaagacggctgtatctgtcagtggaagagtcaggaaccaatcagtattcctcactgtacggatatttcgggtggtccgaccatctggaagtgtcgatg
taacgcatgtttctgttcagcccatcgatgcttctcatgtcttccgcgtccagctgaaagtcaaaaacctggaagttctgctggatcctggctggggtga
agctctttgcaaggacaacgactcctcgctggaggtggtaccgcattgctacctgagcgggggtccggttgtgcttcttagcgatctcagtgagcgcagg
gtcttccaggagcacaggagtgctcgcttctatccatcgctcatccctgctggaccccaatacgctgtatcccaccaacacgatgtccttggacttgcag
aactccagcagtttgctctgattcaggtagacgtggcattccacctggttacagacgggtttatatttcagcccaggcatattgaggatcagctccagct
gcttgtggttaaaattggacaccccgatggatctgaccagccctgcgtctttgcacttttccagagccttccatgtatctctaaagtccggtgttatggt
aaataaatttcccatttttcatccgcccggaaaagagatcatctccagggcttgaactcca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: