XB-CLONE-4351693
Clone Name: IMAGE:7027115
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Gene:
entpd1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784731
[+]
taattaatcccccccccccccggagagcagccaggcacaggctgagacgcaccacacaagctgagctgatggacgaaccaaaggctgtaaaacagaagaa
gacatggcacaaaaaagtcataatcttcctaggagctctgtttgtcttgggtgttatctctttagtcgcaattgcagtagtgcagaataaacctcttcca
aagaatattaagtatggcattgtgctggacgctggttcgtcccataccagtgtgtatatatatgaatggccggcagaaaaggaaaatgacaccggtgttg
tacagcagataaacgagtgcaaagttgaaggcaacggtatatccagttatggccacgagccactgaaggccggtctttctctacagaagtgtatgaataa
agcccgtcaggtcattcctgagaagcagcaaagggagacaccagtttatttaggggccacagcaggaatgcgtttgctcaggctaactaatgcaacaatg
gctgaggaagtcctgtcttcagtggaaaatacgctgcgttcctttccgtttgattttcagggtgccagaataattacaggacaagaaagacagcgcttat
ggatggatcacaattaattatctgctttggaaactttatccaggaatcaggtttggtttcaatatataccaaatttcaaacccactgaaaacttcccggg
gcactggaaccttgggaggtgcttccaaccccgattcaccttttgtaattccaaaaaaaaaaaattggaatccccaaagaaaaaattcccttggcacctt
tccggcccttttatgggaaaaatccctcaggaatattcctataaccccaacaaacctt
3' EST: CF784730
[+]tttttttttttttttaaccgaagttctttttctttatagaaacataattgattttgaatacatcaacaataacaataggttatggcagaagaaggcagca
atagtgctcaagagcgactaccaggattaatggataactttgcctcatagaggacaccctcatgccaatgtgaacctgttcccctagcagtcttagcagt
ttgccaggagattcacagatattaccaaagattcaatgacttttagtgcctgcttgtgtttgtctgtcaaacttgcagtctctggcttcaaagattgggg
tgtgcaaacttcaatggacttcactgtccacctacttaccgaacccataaaaaggagcagtggagcaacgtaaaagaggaaactaaatgtctaaaagcca
aagtgcatgttaatagtcaccgacagtgaatcttgtggagatactcaggggctgatttatgtagcattagcaaatggcaaagtctgcttctttgggagta
gattcacagtataatgaggccatggcaatgtccctaggatttcctttacaagatttgtctaatttgaattcctataataatgcacagttattatatttca
ttgcaattcttttgctgcatatagctttgatcttccatttggtattacaattgctgttgtccactgctttactctgaaggaaggaacctaggtcttcact
gtgtattgtttctaaagatgggggactatgtggaagcntaagaataataaagagctgaagctggggggcagatgcagtagcaatatgtacatgtgcattg
tttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: