XB-CLONE-4351690
Clone Name: IMAGE:7027114
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Gene:
sub1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784733
[+]
taattaatccccccccccccctttttttttttattacgcgctattgtacatttggattgtaaaacaatttgtcaaatattacacgtctgcgctttacaat
ggcattggaatttacaccacgctactatgtaaaatatttttgtttaaaaagtttcccattaaaaatatcattctttaaaacgaatgcatgaaaacacttt
tttctctgatcctgaaagggcagcttaagagttcagtgcaaatgccctgcattttgtatatattaatacgtagggctactgtaccacagccagcagcttt
aggctccttcttttccaacatttctccaccttgatgcccatacacttgtttaattacctggtatggtgctttgcccatttttatttgacctaggcaaaga
gatgggggcagacaacactggagaaacccatgagtgcagtgtaaaccaattggtttgaagcataacaaagccgtcggtattagtataggcagaatgaggc
gaattagtgctttgccttaagttgcccgtgatcatttcagatttaatgatgtttgatttaaagaacaatccatgtactgtgatttattatagggattttg
agcgcctgactgtttctgatgttcctcttacggtttccagcactgcagtaaatgctcagcgtgggggacacggcagggtgtaagaagacattctctgggt
ccttactggccaagctgatttttttcagc
3' EST: CF784732
[+]tttttttttttttttcttttttaaaggtcagggggtaggtattcacatggattagtgtaattcaggaatacccatcgtaaccccctaaggcttgcttttg
cactacaagccccaacatccctgtggatgctcaattctgcatatctgaagaactctttgatgaccaggtcaagagttatcagttctagtaacagctgatt
cctattctgttcaagtaaacaagacctataactttcacagaaaagtttctaacaaataaaaaaaactttctaaatataagcaattaaaaatgtactgttc
ctgaaataattaaattactcttcactcttccctttttagcaatctttctgttttgattgacaggcaagtctagtacctcattggaggggggggggggggt
tacctaaaagatgtattagagctaaccagctctgatggaaaccctgtctctctacatgcaggatttgtgccagtcattttgttagatttttcttgtgcta
gagtcactgttacattttgtttgtgctggagttggctttatgttagctctaatatatctgctaggaaagggagcccccctaaaagatgtattagatctac
ctgccaatcaaaatctgacttctgtatgaagagcaaagagtaaagagaaacttatttcagaaaaggaatagaattttaaaatgattggatttggaaaggt
ttcttattacattgagaaaaaagcttttttttttggggcacggtggaaagtacccaagcccaaaggttaaacatttaaaatggttaaaaaagtgcaaaaa
aaaaaaaggtttaggtat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: