XB-CLONE-4351678
Clone Name: IMAGE:7027109
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Gene:
eno1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784740 [+]
taattaatcccccccccccccgctccccagtcattgccggaagagaacggttgtacctacaagtagaaagtaaaaatgtctatcaagaagatccttgcac
gtgaaatttttgactctcgtggtaacccaacagtggaggttgacctgtacacatgcaaaggtctcttccgtgctgctgttccaagcggagcctccactgg
catttatgaagcactagaactccgggacaatgataagactcgctacatgggaaagggtgtcggaagagctgtcaaatatataaatgagtttctgggacct
gcattatgcacccagaatctaagtgttgtggagcaggaaaagatcgacaacctcatgattgaaatggatgggactgagaataaatccaaatttggtgcta
atgcactcttgggagtgtccctagcagtgtgtaaagctggagctgcagagaaaggagtcccactgtaccgtcatatcgctgacctggctgggaacccaga
ggtcatccttcctgtaccggcatttaatgtgataaaaggggggttctcatgctggaaacaagctggctatgcaagaattcatgatcttgcctgtgggtgc
aaacaccttttaagaagccattgcgcataagaagcagacgttgtttcttcaaccctaaataatgtcatccaaggcctaaatatgggacaaagatgcccac
ctaaaggtttggtggaatgaaaggaaagcctttgcctcccccattattctttggaaaaaaaaagggaaaccccttgggaactttttatagaaaaactggc
ccattaaatccacgggcttggtctttaccccctgtatataagaggttttgttaacttttgtgaaactgggaatggtttttgctc
3' EST: CF784739 [+]tttttttttttttttcgccttcaaagagtttattgcctcttcgtgtctagagtgtgacaaaccattatctagtctgaaactgcaataggatgagaacaca
agactacaaagttgacatgaccaggaccactgccacaatagaaaagacaaggactacatgcaggcaccagtgtgacactgaatttggaataatcctaagc
agcaaagcttttaaactggaaagacagcaaggttatgctgggagaggctgtaacatctaacattgctggaagtgcctgaaattaaatgagggctcagcag
tgacatgtaagacctcctgtagtctctcctaggtgcacagctactacatcagttgggtgagagcctaaggatgcctccatttagttgaaaacaggttttc
tgaaatttcttccagcaaagcgggccttgctgccaagctcttcttcaatcctgagcaattggttgtattttgcaagtcgctcagatctgcatggagcacc
tgtcttgatctgtccagtgcggagaccaaccaccaggtcagcaatgaaagtatcttctgtctccccagagcgatgactgaccatcacaccccagccattt
gactgagccagcttgcacgcctgcagggattctgtcactgtgccgatctggttgaccttcagaagcagacagttacaagacttctcatccacagccttgg
caatacgttttagggttgggtcactgtcaagtcatcaccaactacctgggatattcnaagatgcagtaaaacttttccaggctttccagtcatccg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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