XB-CLONE-4351675
Clone Name: IMAGE:7027108
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Gene:
kdm3a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784742
[+]
taattaatccccccccccccccagaagcattgttgggatgtgccgctacaactccaggcatccaaaatgcagcaacacctcctcaggctaattctcctcc
aagctttggtgcagcaacacctcaggggaaaggaacccagaatttgccaggagatactacagtgctgaatggcgattcaaagagagaagagaccaatttg
tttctgagcgctgcagcctcccagggaaataagaggagcttgggttttgggatgatggaaagtcccagtactttttcatcagtatctgcgatgccttcct
ggtctgggtctggccaagcaaagagtgataatgggcccaaatctgagaacctctttactgcatttacaaaatcaacagtctttccaaagggatttgagtt
ttcagtgaagagtttccctgagcaaaagatgctctctgtgaccgattcccctaaaacttcccccccaaaggtctgcgtgcagcagcagcaagagcaaaaa
gtcgtaaagaaaccagagaacaatcatacaagtgtgaaagctgttcaatctcaagaaccaccttatccaaagagcccaaacaaaaaagatgggtgtaacc
taccccagatccatcctgctgggatccacaggagcctggaaaaagttgcagcaaaaacggggaactgcttttgtgcagggatggctcttgcaatgacatt
tgcaccctcatcttcataaattgccggggaaatgcgcccggggaccgggtttggg
3' EST: CF784741
[+]gtgcagtgcatacattgggctgtgcaaggggtgcctttgggggctctggttcaggagtgctcattttcacttcttcctttacaactggcttaggtaccgg
ccgtacgtgcttgtttgaacacagacaattggactttattccccatcttcccctcacagaatggacaatatcgcctacatcagaaagagctctgcctgga
acgatctgagttggcataagattttctggttcatgcaactggccctttacacatttcaaccatgagaatgactcatttccatcctcaccactgcttaaac
ttttctttttcatcctgtagcagtctacacagacaccaaagccacacttggggcatacccagtgcagattaaatatggttgtgtcacatacatcacacat
ttcacggaccccacgtactgctctcttccaggccacttgttttgatggatcaatggcagacatcacttccttttcggacatgaccagtttgcagaaatgg
tctccaatattagccaggatgtactttgccgtatcaaggtcaaggtccacaacgctgcttgccagagggagccacagatttattgcttctgcatcatact
tattgggagtcaggaagcctccttcctttagcatgccgtgtttgttgaagtgcaaccttcggaaatggaagaacctgcagaacacagcagaatcccgctg
ctccctgcttcggccaaaccggtcccagcggcattccctgcacttatgaagatgaggtgcaatgtcattgcaagagccatcctgcacaaagcagtccccc
gctctgctgcaaacctttttagctttcg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: