XB-CLONE-4351659
Clone Name: IMAGE:7027102
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Gene:
rps7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784752
[+]
taattaatcccccccccccccgcctcttcctaggccgtcactgagacaggacgaaaggccatgttcagcacaagcgctaagatagtaaagccgaatggcg
aaaagccagacgagtttgagtcgggtatttcacaggctctcttggagctggaaatgaactctgaccttaaagctcagttaagggaacttaacatcacagc
tgcaaaggaaatagaagttggaggtggtagaaaagcaattatcatctttgttccagttcctcagctgaaatctttccagaagatccaagtaaggctagtg
cgcgagttggagaagaaatttagtggcaaacatgttgtcttcattgctcagagaaggattctccccaagccaacaagaaaaagccgcacgaaaaacaagc
agaagcgtcccagaagtcgcactctgactgcagtacatgatgccattctagaagacttggtttacccaagtgaaattgtgggcaagaaaatccgtgtcaa
actggacgggagtcggccttatcaggtccatttggaacagggcacaacagaaataatgttgaaacaccagggtggaaaacattttttctgggggggtaac
aaaaaaacttcactgggcccaagaatgtgggttcctttgaaatttcccccgaaaatttccagccctgtaaatggtggccaggaattgccctaaattaaaa
aaggttactttcctttgttttccagggaaaatataaaataaaaaaaggggattcccccctttgtttcctaataagggggcccgccccacccataggtggg
aaaaactttgggcccaggtgtgcccaaccctttttttttatatttgcccagcctttattaaaagagggtttacaaaatattaaaggcgtattgccgcctt
cctctcaaacattttccaacaatatta
3' EST: CF784751
[+]ttttttttttttttctttaacaagaagtactttttattatgcaatcctgcacattacagctggaactctgggaattcaaagaccacatctttgcctgtga
gtttcttgtacacaccagaaaatgtttccaccttgtgttcaacattattctgttgtgccttgtccaaatggaccttgataagccgactgccgtccagttt
gacacggattctcttgcccacaatttcacttgggtaaaccaagtcttctagaatggcatcatgtactgcagtcagagtgcgacttctgggacgcttctgc
ttgtttttcgtgcggctttttcttgttggcttggggagaatccttctctgagcaatgaagacaacatgtttgccactaaatttcttctccaactcgcgca
ctagccttacttggatcttctggaaagatttcagctgaggaactggaacaaagatgataattgcttttctaccacctccaacttctatttcctttgcagc
tgtgatgttaagttcccttaactgagctttaaggtcagagttcatttccagctccaagagagcctgtgaaatacccgactcaaactcgtctggcttttcg
ccattcggctttactatcttagcgcttgtgctgaacatggcctttcgtcctgtctcagtgacggcctnagaaaancnggn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: