XB-CLONE-4351656
Clone Name: IMAGE:7027101
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Gene:
ccnd1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784754
[+]
taattaatcccccccccccgattcccaactttgatctctttctggcaacatcgcgtcactgagccggggagctcggtgcgacataagaaaggctcctgca
gggaagggagcgcaaagacgcgcaaagacgcacggacatggagctgctgtgctgcgaggtggacaccatcaggcgggcccatctggacaggaacctcatc
accgaccgggttctgcagaccatgctcaaagcagaggagacctgctgccccaacgtgtcctatttcaagtgcgtccagaaggagatcctgccccacatgc
gcaaaatagtggccacctggatgctggaggtctgtgaggagcagaagtgcgaagaagaagttttcccccttgctatgaactatttggaccgattcctgtc
cgtgaagaccctcaggaagagccaattgcagttgctgggggccacttgtatgtttctggcttctaaaatgaaggaaacgatcccactgactgcagagaaa
ctttgcatttacacagacaattccatcaggccagaggagttactgctaatggaactgctgattctgaacaagttgaaatggggacttggcctctgtgacc
ccccatgactttattgaaacattttctaaacaaaaatgccactgactggaggataccaagcagattatccgccaaacacggcccagaacatttggttgcc
cctctggcgcctacagaatataaaaggtttatcctcccaaatccacccctccaaggaatcgccccccccggcagttgttttgccaaccaaccccgtttca
agggaaactaaaaatttttggggaaaaacgccccgaacaggtgggtt
3' EST: CF784753
[+]tttttttttttttttgttgtaggaaagtttttgggaattatttgtactttttttttttttttcatagaggtgaacgggtatattttgaagcagaatttcc
tccgggtgtgaccaaagtgttccggaaggcgtggccttgttctcatccgcaagttatatcccaccacactccaaagctatatatatatatatatatatat
atatatatattggagcaaaaaaaaaaatgtctgtgagccgccgtgtcaaatgttgacgtcccggacgtcggtcggggtgcaggaaatgtccgcctcttcc
accatgttttttgtgtctgaggacgtgttgtgttgctgctgtgcttggcgcaaactggactctaggagagactcgatctgttcttgacatgcccgtaaac
agtccgggtcacatttgatgacctgtgatagaaaaagcgtgaggcgctgagtggagaagacactgtcggcgtttcccaaatttagtccctgaacggctgc
tgcaacactgccggcggcgatcatggagggtggattggagataaactttatatctgtagcgcagagggcaacaaatgtctgggcgtgtttgcggatgatc
tgcttggtatcctcagtcagtggcattttgtttagaaaatgttcaataaagtcatggggggtcacagaggccaagtcccatttcaacttgttcagaaatc
acagttccattagcagtaactcctctggcctgatgggattgtctgtgtaaaagcaaaatttctctgcagtcaagggggacgtttcccttcttttaaaagc
caaaaacaatacaagtggccccccagcaaatggaatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: