XB-CLONE-4351653
Clone Name: IMAGE:7027099
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Gene:
sox13
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784756
[+]
taattaatcccccccccccccggcagtttacggacgatactactaatatttggtttgtcagaaaggaggcggcgcggagaataccagatttcctcatatc
agagggtcaggctttgccaagaagtgcttgttgaagatacaacttaaatagcatggttggttaaatggataacttacagggtgagcttgtatcctttcct
gcaagccagcaggagtgcagaatatctgcaaagataccagtctctccaagtgaaggcatagaaggacactgctttaaagtggaggaagattatgaagaac
ctgctttacaatctggcactcccccattcttcactaaacaggattatgaatctccccctcaggatactaatgacttatgcgaacttcaggctgactcttc
tgggtctggcagaaaagaggaaaatctgcagctaagcaaaacagtgagagatgctatgcctgccctagagaagttgctgtctaatgactggagggaaaat
acttctaggtaattctgctgtagaaatcaaagattcaaaggatagtgaaacaacaacaacagctgatccagcaacagcacaagattaatctgttacagca
acagattcagcacgtaaacatgccatatgtgatgattcctgcctttccccctgctcagactgtaacagcagatcccccgatgtcactttcctatacagcc
ctattcccatgcaaacttgtgggaataccccaatggtcactttctttcacaaactccacaaacaaagagggctcttgttatcttgcccaaaacgcccagg
ggaaaacattccccagccc
3' EST: CF784755
[+]ttttcttttttttttcacataaaatgaacagtttattttcttccttcctcaataaagatttagggcacaggaaaataattctggcttaagttacactctg
attaaaaaaaaagttaaaaatgacctttccatgtttacaaaataaaaaaatacatacttacaagtgagtatgtatcttttctatgaggtatgcagataaa
gaggcagccttatccttttccatctatacaataaggcactttggggtataaaaagatgactgttcatctgagttttgacattcactaaagtaggcaacaa
acatggcttcttttattccaaaaaagatccaatcctataaaggtaatcaaaacctatactcctattatgcagatagattattggtatactttaaaatagg
gctgtcgaactggcccactgactgggtgtcaccatccaagaaacttttatgaccaccatcctctgtaaggtctacacagacatcattttatgaaaatcat
ttttacataaactgaccttagaacatgcagaatggcaagtggttgtcccactgcttgcatattgttgcacatcactgtctttgtaatattgtagataata
ttttttatcaagtttccatgtgatacagaccttactagtttaccattagttaacacttaaaatgtgtgacattagcctagtagcatggtgatgcttgggt
gatgtattgtaacagcacttttgttacagacctttaaaggatgtcggtcatccagctgcagcttgctgtactacagatgttaaaacatataagctctatg
cccgtacagaatccctgcttcttaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: