XB-CLONE-4351636
Clone Name: IMAGE:7027091
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Gene:
scamp5
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080466
[+]
GAGTCCGTCCGTGGGATAGAAAGCTGGCTGTCTCTCGCTCTACTTCAACTCCATTTAGCGATTGTTAGGCTTCAACTTAACAGGGGACTTTTCCCTCCTG
ATTGCCGGCACAGACTCTGCTGACCATGGCTGAGAAAGCAAACAACTTTCCTCCACTTCCTCGTTTTATACCACTCAAGCCATGTTTCCACCAAGATTTT
GAGAATGACATCCCAGACCTACATCGGACAACTTGCAAGCGTCTCTACTCCCTATGGATGTTGAATAGCATCACTCTGGGCGTGAATCTTATTGGCTGCT
TGGCGTGGATGATTGGAGGAGGCGGCGCCATTAACTTTGGCCTTGCAATTCTGTGGGTCATCCTCTTTACACCCTGCTCCTATGTCTGCTGGTTCAGACC
AGCTTACAAAGCTTTCAAGACAGACAGCTCATTTAATTTCATGGCTTTCTTCTTCACATTTTCTGCTCAGCTGGTGATCAGTATAATCCAAGCTGTTGGC
ATTCCAGGCTGGGGTGTCTGTGGCTGGATTGCTACAGTCGGCTTCTTTGGCACAAGTGTTGGTGCTGCAGTGGTAATGCTGTTCCCAACCATCTTGTTTA
CTGCTGTGGCTGTCCTCTCTTTTGTGGCACTAACTAAGGTTCATAGGTTTTACCGAGGTGCAGGAGGTAGCCTTAGCAAGGCACAAGAAGAATGGACGAC
GGGAGCCTGGAAGAATCCTCATGTACAGCAGGCAGCTCAGAATGCTGCACAAGGTGCCATGTCGCATAACGACCCTCAGTATTCAGCAACACCAAATTAT
GGCTACTCTAACTAAATGTGAGGCTGTTTAAGGACCAGCAAACAAATATTCCTTGGGAAGAAGGTCCTGCCCTAGTAGTTAACTTGTTTTTTTTGTTTGT
TTTTTAGTCCTCCTGTTCACCTTATATCATGGTGTATAATTGGGTGTGGCTTGTATATATGTTTCCCAGAGAGAATCAGTCTCAGGGTACTGCAGAATTT
GCCTAAGGGAAAAGTTATGTTTACATGCAGATATAAAGTATATAGCTGTTTTAAGCACGGTGTAGGTAAGAACTCCCACACAACTAAATGGCAATGACAG
TTAATTGGGCACTGAACTAAAATAGATTAATATTCCCTTGACATTTAAACTTTAGACATCACTGGTGCTTATCATAAACTAACAGCAGAACAATAATAAT
ACAGTGCTTTTTCCATATACACTGTGGTTAAGCTCAGCCCACTGAGAAGCCCTTTGTATAAATGTACTGCTGATTTGTTTGATTATGCTCATACAAACCT
GAGGTAGATTTTCCCCAATAGGCAAAATATATGTTCAGCACAAGCCCTATTTATTGAACTGATGCTAAAAAAAAAAGTTGTATACAATGGAGATTATGTT
ACCTGTAATCTTTGGGGGCCACTGCTTTACTCCAGTAACCATACTGGATTTAGTAACAGAGAATTTTTTACACTCCGGCATGAAGGATCCATAGAAATAG
TTGGTATTTAACCTAGTTGTTCTTTCTCTCTTTCTAATATCAGTAGCAAATGCTAAAGTTTATAATTCTTGTTATAGCAATACCTACATGTCTACAAACG
GGTACAGGAATACCCGATGCCTAAAAACTGACATTTACTGCCTTATAAAATCTTGCTTCTTTCTGTGTGTGTGTATATATAAATAATTTACTTATATATG
GTTTTATGTTGATGCCCTCTGCCGTTTCTATAATGTTTGTATGACCAACTTCACAGGACCAATTCAGTAGTGACAGTCTGTTTATATCATTAAAAATGTG
AATTATGTTAACTGTTTTAACTTGAATATAAATTTAAAAAAACTAACAAGCTAGGAAATAAAAGAAAGCAGTGTAAAATATAAGATTATTATGGATCTGA
TGTTACGTTATTGCCTGTAATCTTCAAAATATGAATTTTTGGATGCAAAACTAGTGGTATGTGTATTCATAACTGATTATAAACCTACATTAGGAAATAT
CTAAAGGTTATCAAAAATATTTGTAATGATTGCTTTTTATAATTGAGAACTCTTAACAATGTAGATAGATTATTAGGTTAATAAATAAGGCTACTCATTT
TTCAGTATTTTAATTATGGTAACAAATGAAAATTTCTTGGTAATTACAAAAACCTCAAATTAAATCCAAGAAGACTGCAGTCATGCAGACTGGACTATAA
ATGTACAAATGATTCTCATTTTCCTGAGCCATATTCAAAGCTCCCAATGGAAGGGATGTCAGGTTACCAGCTTAGATTTTTAGCTTTGTTTAAAGGGTAA
TATAATATCCTAGTATTTAGGAAATGTGTAAATTGAACTTTGGTAAGTCACAAATGTGACATAAGATGTGATTAAAAACTGAAATAAAAAAATATTTTGA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF784766
[+]taattaatcccccccccccccgagtccgtccgtgggatagaaagctggctgtctctcgctctacttcaactccatttagcgattgttaggcttcaactta
acaggggacttttccctcctgattgccggcacagactctgctgaccatggctgagaaagcaaacaactttcctccacttcctcgttttataccactcaag
ccatgtttccaccaagattttgagaatgacatcccagacctacatcggacaacttgcaagcgtctctactccctatggatgttgaatagcatcactctgg
gcgtgaatcttattggctgcttggcgtggatgattggaggaggcggcgccattaactttggccttgcaattctgtgggtcatcctctttacaccctgctc
ctatgtctgctggttcagaccagcttacaaagctttcaagacagacagctcatttaatttcatggctttcttcttcacattttctgctcagctggtgatc
agtataatccaagctgttggcattccaggctggggtgtctgtggctggattgctacagtcggcttctttggcacaagtgttggtgctgcagtggtaatgc
tgttcccaaccatcttgtttactgctgtggctgtcctctcttttgtggcactaactaaagttcataagtttttaccgaggtgcaagaaggaacccttagc
caagcacaagaaagaatggacgacggggagcctggaaagaatcttcatgtaacagccaggccagcctcaaaaatggctgcacaaagagtgcccatggtcc
ccaaaaacgaaccccttcaataattccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: