XB-CLONE-4351630
Clone Name: IMAGE:7027088
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Gene:
rpl37a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784770
[+]
taattaatcccccccccccccgccttttccctcgggtcctgaagcaagatggccaaacgcaccaagaaggttggcattgtgggtaaatatgggacccgtt
acggtgcttccttaaggaagatggtcaaaaaaattgaaatcagccagcacgccaaatacacctgctcattctgtggcaagaccaagatgaagagaaaggc
tgttggaatctggcactgtggatcttgcatgaaaattgtatctggaggagcctggacctacaacaccacctccgctgtcaaagtcaaatctgccatctga
agactgaaggaactgaaagaccattaaacaccacttgttcttgaaatccgtaatagtccaacgagtattatctggactgagtgtcaagaaaaggtggttt
cggtagagtgaaatcgaaaacgaaaatattagaggatttccactagtttatggaagagggcgggtctaccaagtaaaaggaagattagaaaacgtaggga
accaaaatgtggatttatttggcagggattgtataataggggctatactaaatatttagagtcaggatagtggatgtagcctgcaaaatattttgcagca
aaaagtgagaagggaccgtagtatgttggtatgtcaccgacgatgcggaggtgtgtgctattgatattagtgggtctgtatattatagataagctcacag
agaggaccatgaacgcgctcagttaatagtgggcggatagagaaccccacctctatatttttttgtttacaaagaggataccatgaggcgaganaataat
tatggagcaggagccaaaaaaaaacgtttgaaatatcgtatgatggctatgtaaaaatggggtgacatcgcagacgataactacatgattaggtgggaga
gcattgatctgtgctgagacaaatagttgatactcaccccgcgggggtcgcgggggaaaaaaacgagacggcaagagtagagaaataccccgagtgttgc
tgtgtcgaagataagggagttataccaacaatttgtctggtggaggaggagtaggatactcgtataaacaatttgcacaacatcttcccgtgtcgcgtaa
tctggattagaggagaaaaagagggcggtaaggacagataaaaaaaaaatatatttataatttatctactcagtagaaagtgttggcagatgtaaggcgt
accacacatacaaantatttagaaatttctacatcctagtaatgatacgagtgaggtgcagtgagacaggtgagatgataagtcgacggaatgatggggt
acatgatggaggatagtacatagacntaaggtagttggatagattgaaacatcgtcgagtatcacacatcacagaangtgtatgcgtacaaaaaacaaa
3' EST: CF784769
[+]tttttttttttttttgtttcatttacagaacaaattttattgacaacagtccagaaaaacagatggaatattactggtttcaagaacaagtggtgtttac
tggtctttcagttccttcagtcttctgatggcagatttgactgtgacagcggaggtggtgttgtaggtccaggctcctccagctacaattttcatgcaag
atccacagtgccagattccaacagcctttctcttcatcttggtcttgccacagaatgagcaggtgtatttggcgtgctggctgatttcaatttttttgac
catcttccttaaggaagcaccgtaacgggtcccatatttacccacaatgccaaccttcttggtgcgtttggccatcttgcttcaggacccgagggaaaag
gcgggggggggggggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: