XB-CLONE-4351624
Clone Name: IMAGE:7027086
|
Gene:
med15
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784774
[+]
taattaatcccccccccccccgagtaggaagatggatgcggatagtgactggcggagcgcgcccttcaggcagaagctggtcagccagatagatgaagct
atgaggaaagctggtgttgcgcacaccaagtcaagcaaggagatggagagccacgtgttcttgaaagcaaagacccgtgaggaatacctttctttggttg
ctcgtctcatcattcattttcgagacatccataacaagaaaacccagccagctgttaatgatccaatgaatgctctacagaatttaacaggcactgctgt
agttggtgggcaaggattgggcttgggggtccgtcctcaaggagctcagatgggaggcatgggtggcatggcacagatggcacagcaaatgaacctgcct
ggccagccacaacctggagcatcagggatggcaccacatggaataactggtgtagctgcagggaaccaagccacccaagttacagctgcagcaaattgca
caacaccagcagcagcagttttcagcaacagcagcagcaacagcaccaggcagctcctacagcatcaacaacaacagtttccaatgggcacagcagcaag
caacaccagttttcaggtggcgggctggttgcttgcaaccccaacaaccagccgccactacctacaaacaaccacaacctacgacctgcggggggcaaca
gtcaataagaaatttccagggaagcaactggcagcatgccaacaccatacttaaaaacgttttaacttatcacacggcatggcaaaattcttggcggcca
tccaattttacaaataaggtgtccaggtacagcacatatgttttcaggag
3' EST: CF784773
[+]tcagtatatccaggagactcttcatcttgcttaaatctttctttctctcttcatttttgtcaatcttattgatcattcgtctcagtggctctatgtactt
tgaaagctgcttcagtttatccagatactgttgttcttctgattggttggtgcttgcaggactcataacagaatttgggtttcctggagtattaaggggt
cctggtgagggaacactaaagttctgaggagtgcgtgctgatgctggactctgggaaggctgaggagatggactgggcatgaagctgctgggtgatggag
ctgggccataactgacattagagttgggttgggacattggttgtgatggttgaggggatggttgtggaggaggaggcatagactgtggagtttgcacctg
ctgcacaggggatggtgaggacattacagggatgctgttctgagagacctgtgggatctgctgttctcccaaagggacggtgcttgtaggattggcagga
acagtggatggcgggaagcgtggtcttactggcatcccaccgcgagccataggggcggaaatcatctgccctgctgctaagtgggcttgaactgcttgct
gctgagctgccttgctgaacccgcctggttgtgccctgctgaacccgcttggttgaccttgctgctgaacccacttggttaaacctgcctgctgtgccct
gccagatgtgccttgcttgccgagccctgcttgttttttcccggttacctaaactcttctttgttaacccgagagacggatgctgccttaactgacagag
atatat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: