XB-CLONE-4351621
Clone Name: IMAGE:7027085
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Gene:
hmgb2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784776
[+]
taattaatcccccccccccccggctgaactgtggaaaacaagaggctgtattgcgtccgtaataaaccatttcgctgcgggagcacacacagagcggtcg
gcagttatctttggattgtgtcttttcattcaggcatcatgggcaagggagatcctaacaagccaaggggcaagatgtcgtcctatgcgtactttgtgca
gacgtgcagagaagaacacaagaagaaacacccggactcctctgttaacttcgccgagttctcaaagaagtgctccgagaggtggaagaccatgtctgca
aaagagaaatcaaagtttgaagagatggcaaaaaatgataaagtcagatatgaacgtgagatgaaaacctatataccacctaaaggagagaagaagggaa
agaaaaagaaggatccaaacgcaccaaagaggccaccatctgcctttttcctattctgttctgaacatcgtccccagataaagagtgagagtcctggtct
ttccattggtgacactgctaagaagctaggagaaatgtggtcagagcagactcctaaggataagctaccatatgagcaaaaagcagcccaagctaaagga
gaaataatgagaaagaagtttgctgcctacaaaggcaaagggggaaaaagtgaatgtttggcaaggaaaagtttccaggaaagccccaaacaggttcctt
atataagaaggggcctaaacccgtaaggaaagacggaatgaattgattgaaaaaattgaaggacccaaaacaagatattgaagggaagggaatgtattgg
atgggaatgcgagnggatatgatatggattgtgaaataaaaaaggggccttttttgggctccgtggcgcctggttgaaaataaagaatttgggtgtggct
cttaaaaaaaagccctcaccaatt
3' EST: CF784775
[+]ttttttttttttttttttttttttgggtgggatatataaattttattacaaaaacagctaaaaaaagttagaaaagtacaatgcaccaggtctataaggt
ctaaagaataaatgggtcttgttactaacacactttgctaccattggattcattaaatatgcagaataatcccacatcagaagctcttacactaacagcc
cgtccactacttatattaaaatataggttttccctaacaatcacattacctatacacaattctgtacagttttgtataaccaagctaacaaaatgagctg
cactcgagttcacattcttagcaaaaacaatgggcttcagcacaatttatacagcagacaaagcatttattcatcatcatcctcatcatcatcatcctcc
tcatcctcttcgtcctcatcttcatcatcatcgtcttcctctggttcggccttctttttagaacctgtcggccttcctggaactttcttgccaacatcac
ttttcccctttgccttgtatgcagcaacatctttctcatatttctcctttagcttggctgctttttgctcatatggtagcttatccttaggagtctgctc
tgaccacatttctcctagcttcttagcagtgtcaccaatggaaagaccaggactctcactctttatctggggacgatgttcagaacagaataggaaaaag
gcagatggtggccctctttgggtgcgtttggatcctntcnttttcnttcccttcttcnctcctttaggtggggtatataggtttttcatctcacgttcat
atctgaactttatcatttttttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: