XB-CLONE-4351615
Clone Name: IMAGE:7027082
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Gene:
myl1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784780
[+]
taattaatcccccccccccccgtcccacttctaggctctgggctgaagatcatcctggtcttacctcctccctcaagaaacacttgggctgctttctttt
tggattccttttttatttctaccccttgttaaactaaaaccagcacaatggcacccaagaaggatgtgaagaaacctgaggctaaacccgctgctgcccc
agcacctgcacctgccccagaaccagaaaagcccaaacaacctgcattcgatctgtccagcgtcaagatcgagttttcccaggatcagcaggatgacttc
aaagaggctttcctgctctttgacaggacaggagacagcaaaattgccctcaaccaggtagctgatgtgatgagagctctgggacagaaccccacaaatg
ctgaggtcaagaaggtcctgggcaacccctctgctgaagagatgaacgccaagaagattgagtttgagcagttcctgcccatgatgcaggccgttgccaa
caacaaggaccagggctcctttgaagactttgttgagggtctccgtgtctttgacaaggaaggcaacggcactgttatgggtgctgagcttcgtcacgta
ctggccactctgggtgagaagatgagggaggaggaagtagaagctcttctggcaagtcaggaagactccaacggctgtatcaactatgaagcctttgtga
aacacatcatgtccgtctaagcggatatctcaagaactgtcacaagatgtctcaaaacaccgggagcccagaacttgtacaaaaccagcaactggttaca
actaactatacg
3' EST: CF784779
[+]tttttttttttttttcagtgctccatggtatggtacacagatctgcctagaagatttcgccaccaactttgtcgggggttgcatcgtcctgtttgatccg
ttttaaatattggcagggttcatgaaagtaaatgttgaaattccagtgatgaaaaataaaaaaaaaatggtgggtgtattgtacagttcagttccgtata
gttagttgtaaccagttgctggtttttgtacagttctggtgctcccggtgttctgagacattcttgtgacagttcttgagatatccgcttagacggacat
gatgtgtttcacaaaggcttcatagttgatacagccgttggagtcttcctgacctgccagaagagcttctacttcctcctccctcatcttctcacccaga
gtggccagtacgtgacgaagctcagcacccataacagtgccgttgccttccttgtcaaagacacggagaccctcaacaaagtcttcaaaggagccctggt
ccttgttgttggcaacggcctgcatcatgggcaggaactgctcaaactcaatcttcttggcgttcatctcttcagcagaggggttgcccaggacctttct
tgacctcagcatttgtggggttctgtcccagagctctcatcacatcagctacctggttgagggcaattttgctggtctctgtcctgtcaaaaagcaggaa
aagcctctttgaagtcatccctgctggatcctgggaaaaactcgatccttgaaccctggaacaaatcgaattgcaggttggtttggggctttttctggtt
ctgggggcaggtggcaggtgcttggggccaacaaccggggtttag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: