XB-CLONE-4351612
Clone Name: IMAGE:7027081
|
Gene:
ufc1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784782
[+]
taattaatccccccccccccccgctactccttcaatatggtggatgaggcgactcgccgggtggtgtcggagattccgctgctgaagaccttggcgagcc
cccgggacaaggaagtgtgggtgcatcgtctgaaggaggaatatcaggcccttataaagtacgttgaaaataacacaaatgccgataatgactggttcac
actggactccaataaacacggaaccaggtggtcctgggagtgctggtacattcatgatctgctgagatacgagttcgatgtggaatttgatatcccaggt
acctacctctcagcttgcgcctgaaattgccagctcccgaactgtatgggaaaacttcccatacgtccgttgcgaggtaagatctgtttgactgagctct
tcaagcctctagtggtcttgacgccttcctaagttttggtctgttccaccttagggcgtttggggttcgaccccccttattagccctggacaactcttgg
aatctgtttcaccgattgtacttgttccgtacccccttatcccccgtgtgctacccctcgctttctccctttccgtgtccctttgtttcctggtgtttca
ccgtggttttttccccccgtcggttcgggccgcggagtgcccccgcccctacccgaacctacttccgcttttgttccccccacgctcttatgaataaata
cttacgcgccaacgcgtaggtgtccggttaaattaccccttatttccccccccccctatgttatggatctcttgcgctgtaatttaatccaccggtgttt
ttattttttttccccacccctttttccccccgctttgttttccacccctgtttcccacagtaaaccttcccctaaacccttgttgttctctacatgcttt
atttcgggtccatatttatcctatcgcttgttttccgccccccacacgtgcgatatattgtctaacacccgggcgag
3' EST: CF784781
[+]gatcaacaaacaaggaatggatggggcgcaggatccggcagcggctggggaaccctctggatcagtccatgttcattgggggggttctaggttcacttgg
atttatgttccaccagtcccttctgatcagatccggaatctccacggccagccacgggcccaaccccaaagccatgagatgcgccagcccaaacttcgga
acgtttcttgcccataacggcttgaagtgctcagtcaggcagatcttccctcccctgtacatcttggctgttttcccatccagttccggaatggcaattt
caggcgcagttgaggggtaggtaactgggatatcaaattccacatcgaactcatatctcagcagatcatggatgtaccagcactccccgaaccacctggt
tccttctttattggactccagtctgaaccagtcgttatcggcatttttgttgttttcaacgtactttataagggcctgatattcctccttcagccgctgc
acccacacttccttgtcccgggggctcgccaaggtcttcagcagcggaatctccgacaccacccggcgagtcgcctcatccaccatattgaaagagtagc
ggggggggggggggggataattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: