XB-CLONE-4351609
Clone Name: IMAGE:7027078
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Gene:
psmg2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084517
[+]
CCCCCCCGGAATGTAAAAGTGAAAGATACAGTGCGCCGTCATGTTCGTACCCATAGGCTCTGAGCAGGATTTCCCGAGTGATTATACGCTGCTGCTGCCA
GCCATATCAGTTGGAAATGTTGGGCAATTAGCCATTGATTTGATTATTTCAACTTTGAAAATACCAAAAGTGGGGTATTTTTACACAGATTGCCTTGTAC
CTATGGTTGGAAGCAATCCATATGAAACTGACGAAGAGAATGCAAAGGAACTCTGCACAAACGCTGAAGTGTATGCATTGCCATCCCAGAAACTTGCAGT
TCTCCAGCTAAGATCACTTGTTATAAAGAAAAAATCCAAATCCTTTCGGCAAGCCCTGGTTTCCTGGATTAAAAGATGTGCTTTTGCCAGAGTGATTCTT
CTCTCAAGTTGCCATGCTTATCACAGAGATGATACACAGCTTTTTGGAACACCATTCCGTTACTTGGTCACTCCAGCTTTACAGAAATCTGTGGCAGATG
TGCTAAAAGAACTGGAATGGAAGGAAATGGAAAAGGTGTCTTCTTACCCAGGGCTGAACGATAACGAGAAAAGGGTTTTCATTCCAGGAGGAGGTTTTAC
AAAGCGATTTTATGATGATTGTTGTTTAGAAGACCTTCAAATGGCTGTTGTGTTAAAATTCTGTTCTGAAGGTGACAATGTACCGGATGCCTTTTCTCTA
CTTAATCAAGTCAATGAGTGGCTTCATTTAGTAGCATCAACAAATGGTGATGTCCTTGCCAAGTGGAAGGCACCTGGATCCTGGCAGTTGCTATTTGGTA
GTGGTTTGCCTGCTGCCATCTTCTGATGGATTTGGGTCCTCTTGTGCTGTTCATGAGCTTCCGGTTGCCCCAGCTTAAATGGACTACTTGGCTATGATGG
TTATTTATTTGTTCAATCAATGACAAGTAACAAGTAGTAACTTGTAATTTGGCCTGCTTAGTTTTACACCAAAATATTGAGTGGAAGAGCTGAAAGTAAT
CCTATCCTCTGACACTTTTACATTCTGCTGTGAAATAAAACTTGAAGAAGTGGATACCATTTTATATGCTGACTAAACAAATCTAGGTCTGCGCAGATAC
ACTTGAATGTGCAGCCACTGGGATACTTCAAACAAGGATTACTAATAAGAATACAAGTAACATGTTCTTCAACATGTAATCAACGTTGCTCAGAGTTTTA
GGCCACCAAAATCTCTGGACTCAGCGTACAGATATTTTGAATTAAAGGAGTACAAAAAGGATTTACAGTTTCTGTATTACCGTTTAACATTGAATAGCTA
TTAAAATATTCTATTTTAAACTACTAAAAAAAAAAAAAAAA
5' EST: CF784784
[+]taattaatcccccccccccaacccccccggaatgtaaaagtgaaagatacagtgcgccgtcatgttcgtacccataggctctgagcaggatttcccgagt
gattatacgctgctgctgccagccatatcagttggaaatgttgggcaattagccattgatttgattatttcaactttgaaaataccaaaagtggggtatt
tttacacagattgccttgtacctatggttggaagcaatccatatgaaactgacgaagagaatgcaaaggaactctgcacaaacgctgaagtgtatgcatt
gccatcccagaaacttgcagttctccagctaagatcacttgttataaagaaaaaatccaaatcctttcggcaagccctggtttcctggattaaaagatgt
gcttttgccagagtgattcttctctcaagttgccatgcttatcacagagatgatacacagctttttggaacaccattccgttacttggtcactccagctt
tacagaaatctgtggcagatgtgctaaaagaactggaatggaaggaaatggaaaaggtgtcttcttacccagggctgaacgataacgagaaaagggtttt
cattcccaggaggaggttttacaaagcgattttatggatgattgttggtttaaaagaaccttcaaatggctgttgtgttaaaattctgtttctgaaggtg
acaatgtaccggaagcctttttctctacttaatcaagtcaatgaaggggcttccttttataagcttcaacaaatgggggatg
3' EST: CF784783
[+]tttttttttttttttagtagtttaaaatagaatattttaatagctattcaatgttaaacggtaatacagaaactgtaaatcctttttgtactcctttaat
tcaaaatatctgtacgctgagtccagagattttggtggcctaaaactctgagcaacgttgattacatgttgaagaacatgttacttgtattcttattagt
aatccttgtttgaagtatcccagtggctgcacattcaagtgtatctgcgcagacctagatttgtttagtcagcatataaaatggtatccacttcttcaag
ttttatttcacagcagaatgtaaaagtgtcagaggataggattactttcagctcttccactcaatattttggtgtaaaactaagcaggccaaattacaag
ttactacttgttacttgtcattgattgaacaaataaataaccatcatagccaagtagtccatttaagctggggcaaccggaagctcatgaacagcacaag
aggacccaaatccatcagaagatggcagcaggcaaaccactaccaaatagcaactgccaggatccaggtgccttccacttggcaaggacatcaccatttg
ttgatgctactaaatgaagccactcattgacttgattaagtagagaaaaggcatccggtacattgtcaccttcagaacagaattttaacacaacagccat
tttgaaggtcttctaaacaacaatcatcataaaatcgctttgtaaaacctcctcctggaatgaaaacccttttctcgttatcgttcagccctgggtaaga
aagacactttttcatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: