XB-CLONE-4351595
Clone Name: IMAGE:7027072
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Gene:
tsg101
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784793 [+]
taattaatcccccccccccccgacagcttcagaacttccgggaaagtccaaacaggtagtgggagagtcgggctgtaccatggcgctgtctgaggcccaa
ctcaagaaactgctcgggaagtacaaatacagggacctctcagtgcgggaaattctcaatgtcacctccctctacagagacctcaagcccatcatggact
cctacgtgttcaacgatggctcctcccgggnagttgctgagtctgtccggtaccattcctgttgccctataaaaggggaacacataccaacattccccaa
tctgccctggtgggctccccgggaaaaccttaccccctttcaacccccccccaaccgggttttggtccaaggccccacccaggttccccatgaaaccctt
ccaaaaacagggggaaaaaaccccttttgaattgccccaaacggggaaaaaaattcctatcctccccccggtttatcttggccccccaaaattggggaaa
aaaaacccccggcccgggtttggggaaaattttgcccgggggggaaattttttttccccaaaaaaccccccccccaaatggggggggggggtttttctcg
ggggggggaaaaaaaaaaacccctccccccgggggtttttttttttttccccgccggggttttttaaaagggggggggccccccggggggcccccccccc
ctttaatcccccccccggggtgtg
3' EST: CF784792 [+]tttttttttttttttgaatcagtagccatttaattagaatattaatcagctgattagcatacaggggatttggtgctgcgggcggctcctcccacccacg
gcacagaattcccagcattccctctgttcccacacagagcccacctactgctggtgaataacaagatccgaggggagccccacccactggcgtaatgtta
gtccgcgggggcggtaccatcagtcctcagccccccagggcaccaaacaagatgagctgccccaactgggtctctaggaataagttaacccccatttcta
atgtttgaaggaagagtctggggaggagaaagggaaggggcggggcttcaatacagatcgctcaatcccgccgtcttcctggccttctgcatgagggcgc
ggagctggaactgcttgcgggacaggagccggacgtgcttaaggaaaacatcgaggtcgatgacgccgcgccggagcgcctcccccaggtagaagatggt
gtcctcgatggcgttctcctcggcgtagagattgaggatctgtttatagaggggagcggtggggacgatcacttcatcgatgtctcggtactcggactga
ctctccatcttctccaccaccacccccagctcctcgtccttcttcctcagggtctcaatgttcttatccacctccgtcacttcctgttccaggcgagtga
ccatctcctccagcttctggtggcccttcttcaggtcttcctccgtgcggcttcagggcgttgagctcggcctgggccccggtcatctcctccttcatcc
ctcaatctcagtttgtcactgaccggccgat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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