XB-CLONE-4351587
Clone Name: IMAGE:7027069
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Gene:
XB5811292
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784798
[+]
taattaatcccccccccccccggatttcagaaggatacacagaccaggcacgggatcaaaaaggagagatggctgcaaatatatttcataagaaggaatt
ctctgaagcttatcaagctggcatgattgaagattctccaaaaattaagaatttggttttgtcatatttggaaaaaaagaatgagaagccatttcagttg
gcagttgatgttggctgtgggacagggaggtccactcaggctctggctccatatttccagaaggtgattggaatagatgtaagtgaatcccagttaaatg
tggccagaaaatgtacttcacacaacaacatctattaccagattgcccaagccgaagaactcccactggaagatgcttcagtggatctgattaatgcggg
ccttgctgcccattggttcaatccagagaaatttgtacaggaagcagctcgagttcttaaacatggaggttgtttggccttacattctttttcattggaa
tatgagattcagtataaggaaaagtcagaggaactaactgctgtttttaatgaggcttgtgaaactatttgggaatttaataggaaagttgttggtgaaa
tgaaggctatgtatccagaattattcagagccataccatttgctgataagcagaggattagcagcatctgtgaagtatttctattaactgtccccgaaat
actgagtttccttcaggcaatttgcttgtacaaggagttcctagagcaagacaaagataaggccactgcatttctacaagatttagagaaaaggtttttt
agatattttc
3' EST: CF784797
[+]tttttttttttttttagtctacatttttatttttgcaaaattgaacaaaataacggtgagaaaggaaatttgcctttaagaccattagattttgataaaa
aacaaagtttctattggaattactgatcttatatgatctgaggttttaatgtttttgtagagaggctgaaaataacatttctaaaatattgttaataatt
acagtaattatagtcttctaatggtgcaggtttgacagttgaaggcagtttaaggcttggaggcaagaatacagaaataatgtacatgaaattctagaga
atcgtcacaagctgattcatccaaaatatctaaaaaccttttctctaaatcttgtagaaatgcagtggccttatctttgtcttgctctaggaactccttg
tacaagcaaattgcctgaaggaaactcagtatttcggggacagttaatagaaatacttcacagatgctgctaatcctctgcttatcagcaaatggtatgg
ctctgaataattctggatacatagccttcatttcaccaacaactttcctattaaattcccaaatagtttcacaagcctcattaaaaacagcagttagttc
ctctgacttttccttatactgaatctcatattccaatgaaaaagaatgtaaggccaaacaacctccatgtttaagaactcgagctgcttcctgtacaaat
ttctctggattgaaccaatgggcagcaaggcccgcattaatcagatccactgaagcatctttccagtgggagttcttcggctttgggcaatctg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: