XB-CLONE-4351551
Clone Name: IMAGE:7027053
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Gene:
ubc
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784820
[+]
taattaatcccccccccccccgagttacactttagcaacggtgcagcttggaggtaactggttgagattcgtgcaggaccggatcggcagcaaacatgca
gatatttgtgaaaacgctcactgggaagaccatcacactagaagttgagccaagtgacactattgagaatgtgaagggctaaatacaggacaaagaaagg
cattcttccagattcaccaaggttgatctttgctgggcaacacacttggaaaaaggccggaacctttttccgacttttaccttcccaaaaggaaatcccc
tcctgccatttggggccccccgttttgaaaaaggggggaatgcccaaaattttttgtgtaaagaacccctctggaaggggggaaaaaccccctttccccc
cttttggaggggggttgggcccccccagcgtgtgtcacccccctcttgaaaaaaagtggagaagggggggcgcccaaaattctccccctcccgcaaaaaa
aaaaaagaggagggggggggtttttcctctccccccctctgtgtgcgtgtannaaaaaaaaaaaaaaaaaaatttttttttttttgtcggggcgncgggt
cgcacccttcttttttttaagaattcaagaganagaagggagggggggggccctcttttcttttttttttttgttctgggcacttgttgtaaaaaaaaaa
aaaacacccccccccgcgggcggcgcgttcttcccctccctcnaaagatntctagagggnggggggggggggggagggacagattttttttttttttgtt
ttccacaccccccgcgcggggggggagaaagacgaaacattcttcttctctcccccnntatnggtcgggncgcggtttgtccgcctccccccccacctca
ctatagatcatttatcaagcgcgagatcg
3' EST: CF784819
[+]tttttttttttttgccttcacattctcaatggtatcacttggctctacttcaagagtaatggtcttccctgtcagtgttttcacaaacatttgcatccca
cctctaagacgaagaactaagtgcaaggtggattctttttgaatgttgtagtcagaaagagtgcgaccatcttccagctgcttgcctgcaaagatcaacc
tctgctggtcagggggaatgccttccttatcttggatttttgccttcacattctcaatggtatcacttggctcaacttcaagagtaatggtcttccctgt
cagtgtcttcacaaatatctgcatcccacctctgagacgaagaaccaaatgtagggtggattctttctgaatattgtaatctgacagggttctgccatct
tccagctgcttgcctgcaaagatcaacctctgctggtcagggggaatgccttccttatcttggatttttgcctttacattctcaattgtgtcgctgggct
caacttcaagagtaatggtcttcccagtcagggtcttgacaaaaatctgcatcccacctctgagacgaagaactaagtgcaaggtggattctttttgaat
gttgtagtcagaaagagtgcggccatcttccaactgcttgccagcaaaaatcaatctctgctggtcagggggaaatgccttcttttgtcttggattttgg
ccctttacatttttcaatgggtatccacttgggctcaaacctcaaggggtaaatggttcttcccctgtccagggttcttccaacaaatattctggcatcc
ccaccctctttaaaacgggaggaacccaaattgccgaagtggaatttcctttttgggaaaggttataaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: