XB-CLONE-4351548
Clone Name: IMAGE:7027052
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Gene:
urod
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784822
[+]
taattaatcccccccccccccggtggaagcagcgccgttgtaacaaggaagagctcaaactatatgacagagatgagtatggagcagccagaacacttgc
tgccagaatgcttccctcctctccataatgacacatttctccgtgctgctcgtggtgaggagtgcaaccatgttccagtgtggtgtatgaggcatgcacg
ggcggtatctgccacagtttcgggagacacgcgctggacaggatttctttgcgtcattgtcggagccctgaaatcagctgtgaactcatactgcaacctc
tgcggaggtttcctcatgatgcgtgctatcattttctcagacatcctcgagataacccaggctttgggaaatggagaatccggatggaaccctgggaagg
gaaccttgtatttccatgaaactattacaaaaccccctaaggcaccttgcaacaagtcttaaatgcctcaatctgggatggtaatccacagggaagctgg
ggaaaatggtaatttttcgagcccattcgaacttttaatgcgggttgttaaatattaaaaagtgggaaatggggtggcccacccttgtgatttgtggaac
attttaccgtgaagtggaaaggctaattggtgtaatcattgtgtggacatgaaaactcctatatacaatgttaatttaagtagagtggggacgtgcgagt
aagtgcgtatattcgtgaaactccaagatgttgatatgtcgtctaccccacctaatagacgcgctcaagaaagctgtcgcaccactattaggggatacca
ttggggtgagataacccatataaagaatagtccccctcccgcgttatgcgcctgtagaagtgataaaggttacacgcagtgcatataaaaaatctttgac
tatctcagagaaaaaaagtagatctaatcacttttactcatccgcagagagaaagaaggttccctagcgtatacggggatggggganaaatatattaaat
tttttatgtggcatttgagatggttactctcatacggttagcagtagaacgaagatgcagtgggtggttggggactctctttgtagaggaccaacttttg
ttgtgcattagcgggggagttcttttgtaaagagctcccactcattctcagcacatgagaggactatgtcacctttataggttggagtacatagtatatt
catagatacttttcgcactgcggaagatctcttttcatgtaaattatcactacgccatattactctttttttgagagatgtgcatgtgtttctccgctgt
gaagtaatat
3' EST: CF784821
[+]tttttttttttttttttttttaccatactttattctgtttatttatccgaagttccactgacttgatatatcacaagagcaacacttgatatatactggc
catcccctggcttagctgtatttgttgtgaaaaatatattcatggagtccaccacaccccccaaatgtccaaagtatttcaattacatcattggctttag
tggtcagtccaaaaaacatataggacaaaaaaaaggattagagggcaaacccatggacacctcaactgctggctcttacatcagtccagaatgtttacta
gacttgatccaattaattctccttttgctgtgaatatttgtgcactgctgatataaatgcccccacgtgatctggatccatatctgggtacaaaccatgg
cccagatttgcaatgtacccacgctggccaaagccttctatcatctttttcacagttttttcaatatcctccttgctggcatacaaagcacagggatcca
tattgccctgaagagtcactcgtttgcccgtgcgttcccgagcttcctgtggtcttatggtccagtccaatcctaccacctcatagccagactgggacag
atcctccagtgcatagtgggcatctttagcaaagacaatcatgggcacctgaatcaatccctcagctttcagtttatgttttactctgcgggaaatatct
ctaagataccgggagaaaaaaatccgagaactgctggggtccaaggcagcccgcatgggaactc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: