XB-CLONE-4351540
Clone Name: IMAGE:7027049
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Gene:
ints3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784827
[+]
taattaatcccccccccccccggtagaaggtttccttatggcttcctgtgtgtgctagaaactgggagcaacatggagccgaaaccaaagggcaagttgc
tgatcaccaccggactggatgccaaggatgagctggaagagaggctggagcgctgcatggcgctggtgaactccatgaccaatggcatgtccgagcgcga
ggccaacgacgccctcaatgcttacgtgtgcaagggggccacccagcacgaggaggtgtgcctcggtctcttcgccttggtactgactgagccggcccaa
gtgcaaaagagctaccgggacttggcgttcatcagccgggatggcatgaacatcgttctgaataaaataaaccagctgctcatggagaagtacctgaagc
tgcaggacacgtgtcggacacagctggtgtggctggtgcgggagctggtgaaaagcggtgttttgggcgcagacggcgtgtgtatgaccttcatgaagca
gatagcggggggcgatatatccgccaagaatatctggttggctgagaacgtgctggatattctgatggagcagagggaatgggtgatgaagagcagcatc
ctgatagccatgtcggtgtacacctacctgcgcctcattgtagaccaccatgggacccccccgttgcaggccctgagacagaaagaagtggaaatctgca
tgatgctgctgaagggagcggttttatggactgcttcatgattggacgaagacctgggtccgacttccttcaagaacgttcgccccgcattct
3' EST: CF784826
[+]tttttttttttttttatcagtcaaaagttttattgtgttttaaaatatttctttctttttaaaaaaaaaaaaaaaaccaaacccatcattgcaggagcgg
aacgcggcaagtcccgcccacgaggaacttatttacaatcctgtacgaaaataggaacctttaatgtctgcgtagggtctttttactctccccgcccccc
ccaggttgggtaggaagggggggctgtgtccccggctagtcggtaccccacatacagggagggcacggctagtggccaaaaatcctttctccgtgcctga
aagtggcaacatggctcccaggcgatggtccaactcccccaggttcatggcacgagacgcacaagatggctgccggcccagtggggcccccattcttgct
cagtcttacggtgcagtcccattggtgcaacacacaggccttgcgattggtcagtcgctgtcggaccccacagcggaggatcctttgcgcttcctcttcg
ttggctttggctttgtgtcctcctcttccgacgcgctgctggaagccgactcctcctctgtgttgaggggctgggctgtgtttttcctgctgcgggggct
tgtcattgggcctttccgccggctctttgggggctttgtagaatcctcatactcttctgccagggcaaataggtcactgaacttcatcttgtgggcctcg
tcacagctggcctgcacgtgctggaaggcctgtaggatcggcgtttggctgaaaaagttctgtttggtgttggacaggttgagccgcagaacgtccagat
gttccaggatctgcttccagagtca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: