XB-CLONE-4351537
Clone Name: IMAGE:7027048
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Gene:
dusp23
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080506
[+]
CCGAGAGTGCACTGCTCAGGCTCACACTTACTTCAGAAATGCCTCCAGTGCCGCCTCATAATTTCTCCTGGGTGGAGCCCGGGCTATTGGCCGGCATGGC
GATGCCAAGACTTCCTGCCCACTACGAGTACCTCTGTGAGAATGGCATCCGACACCTGATAACCCTGACAGAGCACAAGCCGCCCTATCACGACACTTGT
CCTGGTATCACTTTACATCGCATTCGCATCCTGGATTTCTGTGCCCCCAGCCTAGAACAGATCAAGAACTTCCTCAAGATCGTGGATGATGCAAAAGCTA
AGGGAGAGGTAAGACCAGTTGCAGTTGGTCTATAACTTGATTAAAGTCCATTCTAAGGTTAAGTCACCCTTTAATAGGCTTTTCCTTCCAGGCAGTCGGG
GTGCACTGCTTACATGGTTTTGGGAGGACAGGTACCATGTTGGCTTGCTACTTGGTGAAGGTCTGGAAGATCACCGGCGTCGATGCCATCAATGAGATCC
GGAGTCTACGTCGGGGCTCCATAGAGACAACTGAGCAGGAAAAAGCCATCATACAGTTCCACCACCACATCAAGTGACCTTCATGGTGTGTCCAGTGGCC
CATTGGCTTGGAGAACCCATGTCTCCCATCTTTCTTCTTCATCCTAGAGGTCAAGGAGGAACTCTAGACTTCTACTGTGTGTGTGTTTGTATTAAATGTA
TCTCAAGTACATCTAGAATGTTGGCCAGTTTCTCCTGCAGTTGCCCAAAATGGGTGACCTGCATGGCGTGTCCAGTTACCCATTGGCTTGAAGAACCCAT
GTCTCCCGTCTTTCGTCTTCATCCCAGAGGTCAAGGAGGAACTCTAGACTTCTACTGTGTGTGTGTTTGTATTAAATGTATCTCAAGTACATCTAGAATG
TTGGCCAGTTTCTCCTGCAGTTGCCCAAAATGAGTGACCTGCATGGTGTGTCCAGTTGCCCATTGGCTTGAAGAACCCTTGTCTCTCATTTTTCTTCTTC
ATCCTAGAGGTCAAGGAGGAGCCCTAGACTTCTACTGTGTGTGTTTGTATTTAATGTATCTCAAGTATATCTAGAACGTTGGTGGATGTTGGCCAGTTTC
TCCTGCAGTTGCCCACAACAGGGGAATAGGGCATTACAATGGCGGATAGCTGAGGGACCTGATTGTGTTTCCAGTTTGGGGGACGGAATGGGAAGCGCTC
AAAGGAAATGGAGGGAATGAGATTTATGTGGAACGTTAAATGCTCAAAGCCGATTGGGTTTCTAGATGAGGAGCAACATGTTCAACTGTTAATGCTGATA
CTGTAGCTCTGGAACACTAGATTGTTCTTCAGCCAATGTTCTGTACCAGCTGCTAGACCTTACTCTCACCCCATAGGGGTTGGATATGGCTGATTATGTT
ATGGGGGGAGGGAAAGACCAGAACCTGGAGTAGTTTGTTGACCCAAACCCACATGTCCCTTGGGTTTCAAGACAACCTGCTTGTTGCATGATGGGAGCTG
TAGTCCATCAGCCACATAGACGGATCATGGGTTCCAGAGCCGGCCAATCACTTGTTGTCTTCATAACCTCAAATCACAGATGTTGGTCCAATTTCACTGG
AAAATAAACCATATTTATAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF784829
[+]taattaatcccccccccccccccgagagtgcactgctcaggctcacacttacttcagaaatgcctccagtgccgcctcataatttctcctgggtggagcc
cgggctattggccggcatggcgatgccaagacttcctgcccactacgagtacctctgtgagaatggcatccgacacctgataaccctgacagagcacaag
ccgccctatcacgacacttgtcctggtatcactttacatcgcattcgcatcctggatttctgtgcccccagcctagaacagatcaagaacttcctcaaga
tcgtggatgatgcaaaagctaagggagaggtaaaaccagttgcagttggtctataacttgattaaagtccattctaaggttaaagtcacccttttaacag
gcttttctttcgcagcaatcgggggtgccctgcattacttgtgttttggggaggacaggttcccatgtttgccttgctacctttggagaaagtcctgtga
aagatcaccccatctttcaatggccttacatttgaaatccctgtaattctaactttggggggcttcccattgggaaaattaaatttgcagtctatggtag
aaaaaccctctttctttatactagttttgcctacacactccaaacaattcataggggttgtaatccatcttccaatgggagtgggtatgttttacgtaga
gataggctacccaagtatttgacactttttgtccaaagaaaaatcccatatagggtgcacaaccctaataagtgtattttctttgaccctagtcgttacc
actagagataagaaactccgtaaatgagaaggaaaatattgtgtgtaggaggaccttattttaaatcttccagattggtttgtcacattgttgtctaaat
aataaaaaagggaaaattctcacaagagcgaacattattctcttatataatatgcgttattaatgctcacaaatctagttattcaagaggtaatatattc
atgtccatacatatacatctagtctttaacacttttcctattagggttccttgatttactatccatatggctcactatattgt
3' EST: CF784828
[+]tttttttttttttttttttttttataaatatggtttattttccagtgaaattggaccaacatctgtgatttgaggttatgaagacaacaagtgattggcc
ggctctggaacccatgatccgtctatgtggctgatggactacagctcccatcatgcaacaagcaggttgtcttgaaacccaagggacatgtgggtttggg
tcaacaaactactccaggttctggtctttccctccccccataacataatcagccatatccaacccctatggggtgagagtaaggtctagcagctggtaca
gaacattggctgaagaacaatctagtgttccagagctacagtatcagcattaacagttgaacatgttgctcctcatctagaaacccaatcggctttgagc
atttaacgttccacataaatctcattccctccatttcctttgagcgcttcccattccgtcccccaaactggaaacacaatcaggtccctcagctatccgc
cattgtaatgccctattcccctgttgtgggcaactgcaggagaaactggccaacatccaccaacgttctagatatacttgagatacattaaatacaaaca
cacacagtagaagtctagggctcctccttgacctctaggatgaagaagaaaaatgagagacaagggttcttcaagccaatgggcaactgggacacacatg
gcagtcactcattttgggcaactgcaggagaaactggccaacattctaaatgtacttgagaaaccatttaaacaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: