XB-CLONE-4351528
Clone Name: IMAGE:7027045
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Gene:
crisp1.7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784835
[+]
taattaatccccccccccccccgagagagaagggacagaaggaaacaggaaaaatgatgctgattgctgtcctctgtctctcagtcttgcagcgagcaag
tgcggcagccgtgtttcccttctcctcactatcaacacgtaacgctaccaacaggcagaagatcgttgatatccacaatgcctacaggagaagcgccaac
ccaactgcatccaacatgctgaaaatgagttggagcattgaggccgaaaacaatgcaaagaattgggcaactacatgcaatcagtatcacagtcaaccgg
ctgcccggcagattgccaatatcacctgcggggaaaatctgttcatgtcgtcctacccggcctcttgggaggaggtgatccaatcgcttcacagtgaata
tgacaattttgaatacggagtaggagccaaggcggttggtctcgtcattgggcattatacccaggttatgtggtacaaatcctaccggattggatgttat
tgcacagaatgccccaacgatggcgtcaggttgaaatactactacgtttgtcagtactacccggcaggaaattatgctgacagaataaattatccataca
aatctggcccgagctgcgcagactgcccagacgcatgtgataacggactatgcaccaatccctgcccgtatgaagatcaatatagcaactgcgatgattt
ggcatctgggagatcaatgtgatacatacccaaatagtaaagggaaggttgccccgggttccctggtctctgtcaaaaacaatggagataatataaacca
ttttcccctt
3' EST: CF784834
[+]tttttttttttttttaaatatttatagtcatgtatttatttgtgttctaattaatagttaacaatgtaacaagcaaaatattccattatattaaaataat
agctcccctttataatgaaattagggaacatgagtaaaaaagaaggctatgggatcccttagccggatagtcttattcttcctttcccagggtgccacag
tcatgtgatttgtgctcagataaacttcagtcacactttactgctgcgctgcaagttggagtgatatcccccccccctcacccccagcagccgatcagca
gaacaatgggaagggagcaagatagcagctcccagtaggtatcagaatagcactcaatagtaagaaatccaaatccggcttgggactcctccagttacat
gggagtaggagaaacaataggttagctgaaagcagttctaatgtgtagcgctggctgaaagctcagactcaggcacaaggcactgagatggcgcctacac
accaatattacagctacaaatacatttgttggtacaagaataaaatgttaaatggcagagggaattatttgctgtgtaacagtgtcatttagaaataaaa
agtaccccataaatatcatgacagtaaggtttggtgatccaaattatgtaaagatccattatccagaaaaccccagtatctagccagtagatattacaca
ggcatacagtagttataaagggttgggaagcctagggaaatggttatattatctcattgtttttgcagagacaggaacccgggcaacccttctttactat
ttggtatgtatcacattgatctccagatgccaaatcatcg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: