XB-CLONE-4351516
Clone Name: IMAGE:7027040
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Gene:
ftmt
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784842 [+]
taattaatcccccccccccccagagagagacacagcgcttcagaagaatcattgtagtttaaggttcctgcttcaacagtgcttggacggaactaaccat
gacttctcaggttcgacagaattaccaccaggaatgtgaggccgccattaatcggcaggttaacatggagctgtacgcctcctatgtgtacttgtccatg
gcctattactttgatcgtgacgacgtggcattgaaaaacttctcaaaatacttcctacatcagtcccatgaagaacgtgagcatgcagagaaactgatga
aaatgcaaaaccagcgcggggggcggatatttctgcaggacgtgaagaaaccagaccgtgatgaatgggcaaatgggctggaagccctagagtgctctct
tcatttggagaagagtgttaatcagtctcttctggagctgcacaagctttctactgaccacaatgatccccatttgtgcgactttctgggagagccatta
cctttgatgaacaagtaaaaattctttgaaaggagctttggaaaatcatatttacccaacctgccccccggaaggggtggctttcccaatccaatgggca
ttggacaaaatttacccgggttttgacaaacccacccctttaagggggaactaccccttgacctgaatcctccccttttttcctggcctttccttcctgt
ttaactggctatccccccctgggaatgtttaaataatcctggtgggttccattttttagttttcccctggccttttttctttggaccccccaataaagag
ttaattttttataaaacgactaattatagcagaaccatcgaacgttaattatcgatagatcttcaatgatttagaacaaggcgaagggggtttcctcctt
cgtgcttttcaaataagccgg
3' EST: CF784841 [+]ttttttttttttttttttttttttttttttttttttttttttttttttttttaaattactttattggggtcaaaaaaagcaaaaaaacaaaatgaccaca
gatattaaactacagggggaagcagtaacaaaaaaaagcaaaaaaagggagatcagtcatggggttcccctaatgtgtgtttgtcaaacaggtactctgc
catgccattgctgggagcacccatccggcgcaggttggtaatatgatctccaagctccttcataaattttacttgttcatcaagggaatggctctccaaa
aagtcccacaaatggggatcattgtggtcagtaaaaagcttgtgcagctccaaaaaaaactgattaacactcttctccaactgaaaaaagcactctaggg
cttccagcccatttgcccattcatcacggtctggtttcttcacgtcctgcaaaaatatcccccccccgcgctggttttgcattttcatcagtttctctgc
atgctcacgttcttcatgggactgatgtaggaagtattttgagaagtttttcaatgccacgtcgtcacgatcaaagtaataggccatggacaagtacaca
taggaggcgtacagctccatgttaacctgccgattaatggcggcctcacattcccggggggaattctgtcgaacctgaaaattcatgggtagttccgtcc
aaacactgttgaagcaggaaccttaaactacaatgattcttctgaaacgctgtgtctctctctgggggggggggggggaattaaattaactccgagggga
aaaagaaacaag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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